Gene transfer to plants by diverse species of bacteria

被引：183

作者：

Broothaerts, W ^{[1
]}

Mitchell, HJ ^{[1
]}

Weir, B ^{[1
]}

Kaines, S ^{[1
]}

Smith, LMA ^{[1
]}

Yang, W ^{[1
]}

Mayer, JE ^{[1
]}

Roa-Rodríguez, C ^{[1
]}

Jefferson, RA ^{[1
]}

机构：

[1] Charles Sturt Univ, Affiliated Res Ctr, CAMBIA, Canberra, ACT 2601, Australia

来源：

NATURE | 2005年 / 433卷 / 7026期

关键词：

D O I：

10.1038/nature03309

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology(1). However, the complexity of the patent landscape(2) has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many public and private organizations worldwide. Here we show that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants. These plant-associated symbiotic bacteria were made competent for gene transfer by acquisition of both a disarmed Ti plasmid and a suitable binary vector. This alternative to Agrobacterium-mediated technology for crop improvement, in addition to affording a versatile 'open source' platform for plant biotechnology, may lead to new uses of natural bacteria-plant interactions to achieve plant transformation.

引用

页码：629 / 633

页数：5

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