Molecular identification of white morphotype strains of endophytic fungi from Pinus tabulaeformis

被引：106

作者：

Guo, LD ^{[1
]}

Huang, GR

Wang, Y

He, WH

Zheng, WH

Hyde, KD

机构：

[1] Chinese Acad Sci, Inst Microbiol, Systemat Mycol & Lichenol Lab, Beijing 100080, Peoples R China

[2] Univ Hong Kong, Dept Ecol & Biodivers, Ctr Res Fungal Divers, Hong Kong, Hong Kong, Peoples R China

来源：

MYCOLOGICAL RESEARCH | 2003年 / 107卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1017/S0953756203007834

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sterile mycelia isolated from Pinus tabulaeformis were grouped into white morphotype strains based on cultural characteristics. Eighteen of the isolates were randomly selected and identified to various taxonomic levels based on nuclear ribosomal DNA (nrDNA) sequence analysis. The 5.8S gene and flanking internal transcribed spacer (ITS1 and ITS2) regions of nrDNA were amplified and sequenced. Phylogenetic analysis of the 5.8S gene sequences indicated that the white morphotype strains were Ascomycota. Further identification was achieved by means of sequence similarity comparison and phylogenetic analysis of the ITS regions. Results showed that strains WMS9 and WMS10 were Lophodermium species (Rhytismataceae), while strains WMS11, WMS13 and WMS18 were species of Rhytismataceae. Strains WMS2, WMS3, WMS4, WMS5 and WMS6 were identified to Rosellinia, strain WMS1 to Entoleuca, and strain WMS14 to Nemania (Xylariaceae). Strains WMS7, WMS8, WMS12, WMS15, WMS16 and WMS17 were xylariaceous species. The potential of using DNA sequence analysis in the identification of endophytic fungi is discussed.

引用

页码：680 / 688

页数：9

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