Molecular identification of white morphotype strains of endophytic fungi from Pinus tabulaeformis

被引：106

作者：

Guo, LD ^{[1
]}

Huang, GR

Wang, Y

He, WH

Zheng, WH

Hyde, KD

机构：

[1] Chinese Acad Sci, Inst Microbiol, Systemat Mycol & Lichenol Lab, Beijing 100080, Peoples R China

[2] Univ Hong Kong, Dept Ecol & Biodivers, Ctr Res Fungal Divers, Hong Kong, Hong Kong, Peoples R China

来源：

MYCOLOGICAL RESEARCH | 2003年 / 107卷

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1017/S0953756203007834

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sterile mycelia isolated from Pinus tabulaeformis were grouped into white morphotype strains based on cultural characteristics. Eighteen of the isolates were randomly selected and identified to various taxonomic levels based on nuclear ribosomal DNA (nrDNA) sequence analysis. The 5.8S gene and flanking internal transcribed spacer (ITS1 and ITS2) regions of nrDNA were amplified and sequenced. Phylogenetic analysis of the 5.8S gene sequences indicated that the white morphotype strains were Ascomycota. Further identification was achieved by means of sequence similarity comparison and phylogenetic analysis of the ITS regions. Results showed that strains WMS9 and WMS10 were Lophodermium species (Rhytismataceae), while strains WMS11, WMS13 and WMS18 were species of Rhytismataceae. Strains WMS2, WMS3, WMS4, WMS5 and WMS6 were identified to Rosellinia, strain WMS1 to Entoleuca, and strain WMS14 to Nemania (Xylariaceae). Strains WMS7, WMS8, WMS12, WMS15, WMS16 and WMS17 were xylariaceous species. The potential of using DNA sequence analysis in the identification of endophytic fungi is discussed.

引用

页码：680 / 688

页数：9

共 52 条

[11] THE ENDOPHYTIC FUNGAL COMMUNITY IN LEAVES OF A COASTAL REDWOOD POPULATION - DIVERSITY AND SPATIAL PATTERNS [J].

ESPINOSAGARCIA, FJ ;

LANGENHEIM, JH .

NEW PHYTOLOGIST, 1990, 116 (01) :89-97

[12]

Fisher P. J., 1993, Sydowia, V45, P338

[13]

FISHER PJ, 1995, NOVA HEDWIGIA, V60, P589

[14] FUNGAL ENDOPHYTES FROM THE LEAVES AND TWIGS OF QUERCUS-ILEX L FROM ENGLAND, MAJORCA AND SWITZERLAND [J].

FISHER, PJ ;

PETRINI, O ;

PETRINI, LE ;

SUTTON, BC .

NEW PHYTOLOGIST, 1994, 127 (01) :133-137

[15] A COMPARATIVE-STUDY OF FUNGAL ENDOPHYTES IN XYLEM AND BARK OF ALNUS SPECIES IN ENGLAND AND SWITZERLAND [J].

FISHER, PJ ;

PETRINI, O .

MYCOLOGICAL RESEARCH, 1990, 94 :313-319

[16] Endophytic fungi associated with palms [J].

Fröhlich, J ;

Hyde, KD ;

Petrini, O .

MYCOLOGICAL RESEARCH, 2000, 104 :1202-1212

[17] Associations between three fungi on pine needles and their variation along a climatic gradient [J].

Gourbière, F ;

van Maanen, A ;

Debouzie, D .

MYCOLOGICAL RESEARCH, 2001, 105 :1101-1109

[18]

Gremmen J, 1959, NOVA HEDWIGIA, V1, P251

[19] Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences [J].

Guo, LD ;

Hyde, KD ;

Liew, ECY .

NEW PHYTOLOGIST, 2000, 147 (03) :617-630

[20] Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences [J].

Guo, LD ;

Hyde, KD ;

Liew, ECY .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 2001, 20 (01) :1-13

← 1 2 3 4 5 6 →