Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1

被引：286

作者：

Herker, Eva ^{[1
,2
,3
]}

Harris, Charles ^{[2
,3
,4
]}

Hernandez, Celine ^{[5
]}

Carpentier, Arnaud ^{[5
]}

Kaehlcke, Katrin ^{[1
]}

Rosenberg, Arielle R. ^{[5
]}

Farese, Robert V., Jr. ^{[2
,3
,4
,6
]}

Ott, Melanie ^{[1
,2
,3
]}

机构：

[1] Univ Calif San Francisco, Gladstone Inst Virol & Immunol, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Ctr Liver, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA

[4] Gladstone Inst Cardiovasc Dis, San Francisco, CA USA

[5] Univ Paris 05, EA Virol Hepatite C 4474, Paris, France

[6] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

来源：

NATURE MEDICINE | 2010年 / 16卷 / 11期

基金：

美国国家卫生研究院;

关键词：

LIPID DROPLETS; CORE PROTEIN; CELL-CULTURE; VIRAL GENOME; REPLICATION; LOCALIZATION; SECRETION; DGAT; MICE;

D O I：

10.1038/nm.2238

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Hepatitis C virus (HCV) infection is closely tied to the lipid metabolism of liver cells. Here we identify the triglyceride-synthesizing enzyme diacylglycerol acyltransferase-1 (DGAT1) as a key host factor for HCV infection. DGAT1 interacts with the viral nucleocapsid core and is required for the trafficking of core to lipid droplets. Inhibition of DGAT1 activity or RNAi-mediated knockdown of DGAT1 severely impairs infectious virion production, implicating DGAT1 as a new target for antiviral therapy.

引用

页码：1295 / 1298

页数：4

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