Molecular cloning and functional expression of the cytochrome p450 11B-hydroxylase of the guinea pig

被引：18

作者：

Bulow, HE

Mobius, K

Bahr, V

Bernhardt, R

机构：

[1] UNIV SAARBRUCKEN,FACHBEREICH BIOCHEM,D-66041 SAARBRUCKEN,GERMANY

[2] MAX DELBRUCK CENTRUM MOLEK MED,D-13122 BERLIN,GERMANY

[3] FREE UNIV BERLIN,UNIV KLINIKUM BENJAMIN FRANKLIN,ABT EXPT ENDOKRINOL,D-12200 BERLIN,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 221卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.0591

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA library has been constructed from adrenal glands of guinea pigs receiving a low sodium/high potassium diet. From this library a complementary DNA encoding an 11 beta-hydroxylase (EC 1.14.15.4) was isolated. Northern blot analysis revealed a major transcript of 2.2 kb size. The cDNA was cloned into an eucaryotic expression vector and the activity of the enzyme towards various substrates was investigated after expression in COS-1 cells. The enzyme was able to perform 11 beta-hydroxylations of 11-deoxycorticosterone, 11-deoxycortisol and androstendione as well as 18-hydroxylations and 19-hydroxylations to a small extent. Phylogenetic analyses with thirteen protein sequences of the CYP11B subfamily showed an early divergence of the guinea pig, but could not establish a definite branching order. (C) 1996 Academic Press, Inc.

引用

页码：304 / 312

页数：9

共 36 条

[1] CLONING AND EXPRESSION ANALYSIS OF A CYTOCHROME P-450(11-BETA) CDNA IN SHEEP [J].