Road to precision: recombinase-based targeting technologies for genome engineering

被引：118

作者：

Nov, Dagmar ^{[1
]}

Gama-Norton, Leonor

Riemer, Pamela

Sandhu, Upneet

Schucht, Roland

Hauser, Hansjorg

机构：

[1] Helmholtz Ctr Infect Res, Dept Gene Regulat & Differentiat & Model Syst, D-38124 Braunschweig, Germany

[2] Inst Expt Biol, Inst Tecnol Quim & Biol, Oeiras, Portugal

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2007年 / 18卷 / 05期

关键词：

D O I：

10.1016/j.copbio.2007.07.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the past years, recombinase-based approaches for integrating transgenes into defined chromosomal loci of mammalian cells have) gained increasing attention. This method is attractive since it enables to precisely integrate transgenes of interest into pre-defined integration sites, thereby allowing to predict the expression properties of a genetically manipulated cell. This review focuses on the current state of targeting strategies including RMCE employing site-specific recombinases such as Cre, Flp and phi C31. In particular, applications for protein expression, virus production, transgenic animals and chromosome engineering are described.

引用

页码：411 / 419

页数：9

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