Genetic targeting of the endoderm with Claudin-6CreER

被引：50

作者：

Anderson, William J. ^{[1
]}

Zhou, Qiao ^{[1
]}

Alcalde, Victor ^{[1
]}

Kaneko, Osamu F. ^{[1
]}

Blank, Leah J. ^{[1
]}

Sherwood, Richard I. ^{[1
]}

Guseh, J. Sawalla ^{[1
]}

Rajagopal, Jayaraj ^{[1
]}

Melton, Douglas A. ^{[1
]}

机构：

[1] Harvard Univ, Harvard Stem Cell Inst, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

来源：

DEVELOPMENTAL DYNAMICS | 2008年 / 237卷 / 02期

关键词：

endoderm; Cldn6; Cre-ER; embryonic stem cells; beta cells; organogenesis; gene targeting; lineage analysis;

D O I：

10.1002/dvdy.21437

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

A full description of the ontogeny of the p cell would guide efforts to generate P cells from embryonic stem cells (ESCs). The first step requires an understanding of definitive endoderm: the genes and signals responsible, for its specification, proliferation, and patterning. This report describes a global marker of definitive endoderm, Claudin-6 (Cldn6). We report its expression in early development with particular attention to definitive endoderm derivatives. To create a genetic system to drive gene expression throughout the definitive endoderm with both spatial and temporal control, we target the endogenous locus with an inducible Cre recombinase (Cre-ERT2) Cassette. Cldn6 null mice are viable and fertile with no obvious phenotypic. abnormalities. We also report a lineage analysis of the fate of Cldn6-expressing embryonic cells, which is relevant to the development of the pancreas, lung, and liver.

引用

页码：504 / 512

页数：9

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