Determination of 3-deoxy-D-arabino-heptulosonate 7-phosphate productivity and yield from glucose in Escherichia coli devoid of the glucose phosphotransferase transport system

被引:49
作者
Báez, JL [1 ]
Bolívar, F [1 ]
Gosset, G [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Mol Microbiol, Cuernavaca 62250, Morelos, Mexico
关键词
amino acids; aromatic compounds; metabolic engineering; glucose phosphotransferase transport system; phosphoenolpyruvate;
D O I
10.1002/bit.1088
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of inactivation of the glucose phosphotransferase transport system (PTS) on 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) productivity and yield from glucose in Escherichia coli is reported. Strains used in this study were the PTS+ PB103 and its PTS- glucose(+) derivative NF9. Their aroB(-) derivatives PB103B and NF9B were constructed to allow accurate measurement of total carbon flow into the aromatic pathway. The measured specific rates of DAHP synthesis were 0.55 and 0.94 mmol/g-dcw . h and the DAHP molar yields from glucose were 0.43 and 0.71 mol/mol for the PTS+ aroB(-) and the PTS- glucose(+) aroB(-) strains, respectively. For the latter strain, this value represents 83% of the maximum theoretical yield for DAHP synthesis from glucose. (C) 2001 John Wiley & Sons, Inc.
引用
收藏
页码:530 / 535
页数:6
相关论文
共 21 条
[1]   IMPROVED ANTIBIOTIC-RESISTANCE GENE CASSETTES AND OMEGA-ELEMENTS FOR ESCHERICHIA-COLI VECTOR CONSTRUCTION AND IN-VITRO DELETION INSERTION MUTAGENESIS [J].
ALEXEYEV, MF ;
SHOKOLENKO, IN ;
CROUGHAN, TP .
GENE, 1995, 160 (01) :63-67
[2]   CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].
BOLIVAR, F ;
RODRIGUEZ, RL ;
GREENE, PJ ;
BETLACH, MC ;
HEYNEKER, HL ;
BOYER, HW ;
CROSA, JH ;
FALKOW, S .
GENE, 1977, 2 (02) :95-113
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   PHOSPHORYLATION OF D-GLUCOSE IN ESCHERICHIA-COLI MUTANTS DEFECTIVE IN GLUCOSEPHOSPHOTRANSFERASE, MANNOSEPHOSPHOTRANSFERASE, AND GLUCOKINASE [J].
CURTIS, SJ ;
EPSTEIN, W .
JOURNAL OF BACTERIOLOGY, 1975, 122 (03) :1189-1199
[5]   BIOCATALYTIC SYNTHESIS OF AROMATICS FROM D-GLUCOSE - THE ROLE OF TRANSKETOLASE [J].
DRATHS, KM ;
POMPLIANO, DL ;
CONLEY, DL ;
FROST, JW ;
BERRY, A ;
DISBROW, GL ;
STAVERSKY, RJ ;
LIEVENSE, JC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) :3956-3962
[6]   Pathway engineering for the production of aromatic compounds in Escherichia coli [J].
Flores, N ;
Xiao, J ;
Berry, A ;
Bolivar, F ;
Valle, F .
NATURE BIOTECHNOLOGY, 1996, 14 (05) :620-623
[7]   CORRELATION OF THEORETICAL AND EXPERIMENTAL YIELDS OF PHENYLALANINE FROM NON-GROWING CELLS OF A REC ESCHERICHIA-COLI STRAIN [J].
FORBERG, C ;
ELIAESON, T ;
HAGGSTROM, L .
JOURNAL OF BIOTECHNOLOGY, 1988, 7 (04) :319-332
[8]   BIOCATALYTIC SYNTHESES OF AROMATICS FROM D-GLUCOSE - RENEWABLE MICROBIAL SOURCES OF AROMATIC-COMPOUNDS [J].
FROST, JW ;
DRATHS, KM .
ANNUAL REVIEW OF MICROBIOLOGY, 1995, 49 :557-579
[9]  
FROST JW, 1994, NEW J CHEM, V18, P341
[10]   A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli [J].
Gosset, G ;
YongXiao, J ;
Berry, A .
JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1996, 17 (01) :47-52