Laser-assisted microinjection into targeted animal cells

被引：54

作者：

Mohanty, SK ^{[1
]}

Sharma, M ^{[1
]}

Gupta, PK ^{[1
]}

机构：

[1] Ctr Adv Technol, Biomed Applicat Sect, Indore 452013, India

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 11期

关键词：

microinjection; optoporation; photodynamic treatment; transfection;

D O I：

10.1023/A:1024038609045

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A pulsed (17 nanoseconds) Nd: YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.

引用

页码：895 / 899

页数：5

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