Separation of proteins including metallothionein in cerebrospinal fluid by size exclusion HPLC and determination of trace elements by HR-ICP-MS

被引：28

作者：

Gellein, Kristin

Roos, Per M.

Evje, Lars

Vesterberge, Olof

Flaten, Trond Peder

Nordberg, Monica

Syversen, Tore ^{[1
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Neurosci, NO-7489 Trondheim, Norway

[2] Norwegian Univ Sci & Technol, Dept Chem, NO-7491 Trondheim, Norway

[3] Ullevaal Univ Hosp, Dept Neurol, Oslo, Norway

[4] Karolinska Inst, Inst Environm Med, SE-17177 Stockholm, Sweden

[5] Uppsala Univ, Fac Nat Sci, SE-75105 Uppsala, Sweden

来源：

BRAIN RESEARCH | 2007年 / 1174卷

关键词：

trace element; protein; cerebrospinal fluid; SEC-HPLC; ICP-MS; metallothionein;

D O I：

10.1016/j.brainres.2007.08.004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A method to study the protein binding patterns of trace elements in human cerebrospinal fluid (CSF) is described. Proteins in CSF samples were separated by size exclusion chromatography combined with high performance liquid chromatography (SEC-HPLC). The column was calibrated to separate proteins in the molecular weight range 6-70 kDa. Fractions were then analyzed off-line for trace elements using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). We were able to accurately determine more than 10 elements of clinical interest in the CSF fractions. Results are presented for Cd, Mn, Fe, Pb, Cu and Zn. The total concentrations of 16 trace elements in human plasma and CSF are also presented. The method was able to differentiate the relative contribution of metallothionein and other proteins towards metal binding in human CSF. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：136 / 142

页数：7

共 21 条

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