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Separation of proteins including metallothionein in cerebrospinal fluid by size exclusion HPLC and determination of trace elements by HR-ICP-MS

被引：28
作者：
Gellein, Kristin
Roos, Per M.
Evje, Lars
Vesterberge, Olof
Flaten, Trond Peder
Nordberg, Monica
Syversen, Tore [1 ]
机构：
[1] Norwegian Univ Sci & Technol, Dept Neurosci, NO-7489 Trondheim, Norway
[2] Norwegian Univ Sci & Technol, Dept Chem, NO-7491 Trondheim, Norway
[3] Ullevaal Univ Hosp, Dept Neurol, Oslo, Norway
[4] Karolinska Inst, Inst Environm Med, SE-17177 Stockholm, Sweden
[5] Uppsala Univ, Fac Nat Sci, SE-75105 Uppsala, Sweden
来源：
BRAIN RESEARCH | 2007年 / 1174卷
关键词：
trace element; protein; cerebrospinal fluid; SEC-HPLC; ICP-MS; metallothionein;
D O I：
10.1016/j.brainres.2007.08.004
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
A method to study the protein binding patterns of trace elements in human cerebrospinal fluid (CSF) is described. Proteins in CSF samples were separated by size exclusion chromatography combined with high performance liquid chromatography (SEC-HPLC). The column was calibrated to separate proteins in the molecular weight range 6-70 kDa. Fractions were then analyzed off-line for trace elements using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). We were able to accurately determine more than 10 elements of clinical interest in the CSF fractions. Results are presented for Cd, Mn, Fe, Pb, Cu and Zn. The total concentrations of 16 trace elements in human plasma and CSF are also presented. The method was able to differentiate the relative contribution of metallothionein and other proteins towards metal binding in human CSF. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：136 / 142
页数：7
相关论文
共 21 条
[11]   Hyphenated techniques for the characterization and quantification of metallothionein isoforms [J].
Prange, A ;
Schaumlöffel, D .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 373 (06) :441-453
[12]   Speciation analysis of trace elements in the brains of individuals with Alzheimer's disease with special emphasis on metallothioneins [J].
Richarz, AN ;
Brätter, P .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 372 (03) :412-417
[13]   Improved multi-elemental analyses by inductively coupled plasma-sector field mass spectrometry through methane addition to the plasma [J].
Rodushkin, I ;
Nordlund, P ;
Engström, E ;
Baxter, DC .
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2005, 20 (11) :1250-1255
[14]   Determination of low-abundance elements at ultra-trace levels in urine and serum by inductively coupled plasma-sector field mass spectrometry [J].
Rodushkin, I ;
Engström, E ;
Stenberg, A ;
Baxter, DC .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2004, 380 (02) :247-257
[15]   Assessment of the contamination from devices used for sampling and storage of whole blood and serum for element analysis [J].
Rodushkin, I ;
Ödman, F .
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, 2001, 15 (01) :40-45
[16]   Metals in motor neuron diseases [J].
Roos, Per M. ;
Vesterberg, Olof ;
Nordberg, Monica .
EXPERIMENTAL BIOLOGY AND MEDICINE, 2006, 231 (09) :1481-1487
[17]   Towards a high resolution separation of human cerebrospinal fluid [J].
Sickman, A ;
Dormeyer, W ;
Wortelkamp, S ;
Woitalla, D ;
Kuhn, W ;
Meyer, HE .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 771 (1-2) :167-196
[18]  
Smith Q., 1997, Metals and Oxidative Damage in Neurological Disorders, P113
[19]  
STRYER L, 1997, PROTEIN FOLDING DESI, P417
[20]   Measurement of trace elements in proteins extracted from liver by size exclusion chromatography-inductively coupled plasma-mass spectrometry with a magnetic sector mass spectrometer [J].
Wang, J ;
Dreessen, D ;
Wiederin, DR ;
Houk, RS .
ANALYTICAL BIOCHEMISTRY, 2001, 288 (01) :89-96
123