Identification of RNA modification enzymes using sequence homology

被引：4

作者：

Ansmant, I ^{[1
]}

Motorin, Y ^{[1
]}

机构：

[1] Univ Nancy 1, UMR 7567 CNRS, Lab Maturat ARN & Enzymol Mol, Fac Sci, F-54506 Vandoeuvre Les Nancy, France

来源：

MOLECULAR BIOLOGY | 2001年 / 35卷 / 02期

关键词：

RNA modification; sequence homology; pseudouridine; inosine; N-2; N-2-dimethylguansine; 2 '-O-methylguanosine; 5-methylcytosine homology;

D O I：

10.1023/A:1010487417460

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Modified nucleotides possessing diverse chemical structures are universally present in all cell RNAs. Their formation is a posttranscriptional process catalyzed by various RNA modification enzymes. Despite the large body of data concerning the localization of modified nucleotides in different RNAs, the enzymes involved in their biosynthesis remain largely unknown. In this review we discuss the recent achievements in application of the sequence homology approach to searching for and identifying RNA modification enzymes such as RNA-pseudouridine synthases, RNA-methyltransferases, and RNA-inosine synthases (adenosine deaminases).

引用

页码：206 / 223

页数：18

共 81 条

[1]

Agris PF, 1996, PROG NUCLEIC ACID RE, V53, P79, DOI 10.1016/S0079-6603(08)60143-9

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].