Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber .2. Pathobiologic responses

被引:10
作者
Gelzleichter, TR [1 ]
Bermudez, E [1 ]
Mangum, JB [1 ]
Wong, BA [1 ]
Moss, OR [1 ]
Everitt, JI [1 ]
机构
[1] CHEM IND INST TOXICOL, RES TRIANGLE PK, NC 27709 USA
来源
FUNDAMENTAL AND APPLIED TOXICOLOGY | 1996年 / 30卷 / 01期
关键词
D O I
10.1006/faat.1996.0041
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The pleura is a target site for toxic effects induced by a variety of fibrous particulates, including both natural mineral and manmade vitreous fibers. We examined selected cytological and biochemical indicators of inflammation in both the pleural compartment and pulmonary parenchyma in F344 rats following inhalation of RCF-1, a kaolin-based ceramic fiber. Male F344 rats were exposed by inhalation to 89 mg/m(3) (2645 WHO fibers/cc) RCF-1 6 hr/day for 5 consecutive days. In lung parenchyma, cytological and biochemical inflammatory responses occurred rapidly following exposure. In contrast, pleural responses were delayed in onset and of a much smaller magnitude than those observed in lung. At both Day 1 and Day 28 postexposure, increased quantities of lactate dehydrogenase, N-acetyl glucosaminidase, alkaline phosphatase, albumin, and neutrophils were present in bronchoalveolar lavage fluid. These responses were attenuated at the latter time point. No significant responses were detected in pleural lavage fluid until 28 days following exposure, at which time elevated numbers of macrophages and eosinophils, but not neutrophils, were observed. Increased albumin and fibronectin were also observed in PLF at this latter time point. These findings demonstrate that the onset of pleural and pulmonary responses following inhalation of RCF-1 are temporally separated and that pleural injury may increase in severity with time following exposure. The increase in severity of pleural inflammation found in the postexposure period cannot be readily explained by fiber translocation. (C) 1996 Society of Toxicology
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页码:39 / 46
页数:8
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