Detection of CAG repeat DNA sequences by pyrene-functionalized pyrrole-imidazole polyamides

被引：20

作者：

Bando, Toshikazu ^{[1
]}

Fujimoto, Jun ^{[1
]}

Minoshima, Masafumi ^{[1
]}

Shinohara, Ken-ichi ^{[1
]}

Sasaki, Shunta ^{[1
]}

Kashiwazaki, Gengo ^{[1
]}

Mizumura, Masatoshi ^{[1
]}

Sugiyama, Hiroshi ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Chem, Kyoto 6068502, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2007年 / 15卷 / 22期

关键词：

pyrene; excimer; Py-Im polyamide; CAG repeat;

D O I：

10.1016/j.bmc.2007.07.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five N-methylpyrrole-N-methylimidazole (Py-Im) polyamides possessing a fluorescent pyrene were synthesized by Fmoc solid-phase synthesis using Py/Im monomers and pyrenylbutyl-pyrrole monomer compound 9. The steady state fluorescence of conjugates 1-5 was examined in the presence and absence of (CAG)(12)-containing oligodeoxynucleotides (ODNs) 1 and 2. Of the conjugates, conjugate I showed no background emission around 470 nm in the absence of ODNs, and a clear increase of emission at 475 run was observed upon addition of ODNs I and 2. The emission of conjugate I at 475 nm increased linearly with the concentration of ODN and the number of CAG repeats. The results indicate that conjugate I efficiently forms a pyrene excimer upon binding in the minor groove of DNA. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：6937 / 6942

页数：6

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