Optimization of medium composition by response surface methodology for the production of tartaric acid by Gluconobacter suboxydans

被引：17

作者：

Mantha, D ^{[1
]}

Basha, ZA ^{[1
]}

Panda, T ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Tamil Nadu, India

来源：

BIOPROCESS ENGINEERING | 1998年 / 19卷 / 04期

关键词：

Fermentation; Combine Effect; Yeast Extract; Response Surface; Sorbitol;

D O I：

10.1007/PL00009020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The combined effect of sorbitol and yeast extract of the medium on tartaric acid production by Gluconobacter suboxydans, NCIM 2049, was studied in batch fermentation while keeping the temperature (30 degrees C) and pH (6.2) constant. Response surface methodology was used to obtain quadratic models for the production of tartaric acid. The multiple coefficients of regression between 0.8945 and 0.9820 was obtained during the process. The optimum medium composition comprising 20 kg/m(3) sorbitol and 2 kg/m(3) yeast extract was verified experimentally by observing the variation of cell mass and tartaric acid production with time.

引用

页码：285 / 288

页数：4

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