Chiral separation of amines by high-performance liquid chromatography using polysaccharide stationary phases and acidic additives

被引：53

作者：

Stringham, RW ^{[1
]}

Ye, YK ^{[1
]}

机构：

[1] Chiral Technol Inc, W Chester, PA 19380 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1101卷 / 1-2期

关键词：

chiral HPLC; mobile phase additives; ethanesulfonic acid;

D O I：

10.1016/j.chroma.2005.09.065

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A dramatic and beneficial effect of ethanesulfonic acid (ESA) on the chiral HPLC separation of basic compounds was found. Using a single chiral column and a starting mobile phase, more than half of a diverse set of amines was baseline separated. Changing alcohol content and alcohol type increased the success rate. Methanesulfonic acid (MSA) proved even more successful. The mechanism of this unexpected finding appears to be a combination of ion-pair salt formation in the mobile phase and increased binding with the chiral stationary phase (CSP) arising from a localized decrease in pH. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：86 / 93

页数：8

共 19 条

[1] STEREOSELECTIVE EFFECTS IN THE SEPARATION OF ENANTIOMERS OF OMEPRAZOLE AND OTHER SUBSTITUTED BENZIMIDAZOLES ON DIFFERENT CHIRAL STATIONARY PHASES [J].