Large-Scale Production and Purification of Ascochyta caulina Phytotoxins and a New HPLC Method for their Analysis

被引：8

作者：

Avolio, Fabiana ^{[1
]}

Andolfi, Anna ^{[1
]}

Zonno, Maria Chiara ^{[2
]}

Boari, Angela ^{[2
]}

Cimmino, Alessio ^{[1
]}

Vurro, Maurizio ^{[2
]}

Evidente, Antonio ^{[1
,3
]}

机构：

[1] Univ Naples Federico II, Dipartimento Sci Suolo Pianta Ambiente & Prod Ani, I-80055 Portici, Italy

[2] CNR, Ist Sci Prod Alimentari, I-70126 Bari, Italy

[3] CNR, Ist Chim Biomol, Pozzuoli, Italy

来源：

CHROMATOGRAPHIA | 2011年 / 74卷 / 7-8期

关键词：

HPLC method; Ascochyta caulina; Phytotoxins; Natural herbicides; Large-scale production; CHENOPODIUM-ALBUM; METABOLITE;

D O I：

10.1007/s10337-011-2115-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The toxins produced by Ascochyta caulina have potential as a natural herbicide. They are three basic non-protein amino acids. Considering the need to optimize and maximize their production by fermentation in order to obtain large amounts of toxins, a new, simple, and inexpensive HPLC method was developed. This new process allows rapid evaluation of the toxin content in the fungal culture filtrates. The simplification and scaling up of the entire process include a single-step purification of the filtrates by cationic exchange chromatography by using a reusable resin with a high exchange capacity and distilled water and dilute ammonia as eluents. This process allows the fast and easy purification of large volumes of filtrates and the harvest of large amounts of a mixture containing the three pure toxins. These methods are described here and their potential to be scaled up to a pre-industrial level is discussed.

引用

页码：633 / 638

页数：6

共 12 条

[1] Effects of the aglycone of ascaulitoxin on amino acid metabolism in Lemna paucicostata [J].