AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
|
1998年
/
275卷
/
06期
关键词:
food restriction;
insulin signaling;
D O I:
10.1152/ajpendo.1998.275.6.E957
中图分类号:
R5 [内科学];
学科分类号:
1002 ;
100201 ;
摘要:
Reduced calorie intake [calorie restriction (CR); 60% of ad libitum (AL)] leads to enhanced glucose transport without altering total GLUT-4 glucose transporter abundance in skeletal muscle. Therefore, we tested the hypothesis that CR (20 days) alters the subcellular distribution of GLUT-I. Cell surface GLUT-4 content was higher in insulin-stimulated epitrochlearis muscles from CR vs. AL rats. The magnitude of this increase was similar to the CR-induced increase in glucose transport, and GLUT-4 activity (glucose transport rate divided by cell surface GLUT-4) was unaffected by diet. The CR effect was specific to the insulin-mediated pathway, as evidenced by the observations that basal glucose transport and cell surface GLUT-4 content, as well as hypoxia-stimulated glucose transport, were unchanged by diet. CR did not alter insulin's stimulation of insulin receptor substrate (IRS)-1-associated phosphatidylinositol 3-kinase (PI3K) activity. Muscle abundance of IRS-2 and p85 subunit of PI3K were unaltered by diet, but IRS-1 content was lower in CR vs. AL. These data demonstrate that, despite IRS-1-PI3K activity similar to AL, CR specifically increases insulin's activation of glucose transport by enhancing the steady-state proportion of GLUT-4 residing on the cell surface.
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Withers, DJ
;
Gutierrez, JS
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Gutierrez, JS
;
Towery, H
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Towery, H
;
Burks, DJ
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Burks, DJ
;
Ren, JM
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Ren, JM
;
Previs, S
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Previs, S
;
Zhang, YT
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Zhang, YT
;
Bernal, D
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Bernal, D
;
Pons, S
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Pons, S
;
Shulman, GI
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Shulman, GI
;
Bonner-Weir, S
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Bonner-Weir, S
;
White, MF
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USAHarvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Withers, DJ
;
Gutierrez, JS
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Gutierrez, JS
;
Towery, H
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Towery, H
;
Burks, DJ
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Burks, DJ
;
Ren, JM
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Ren, JM
;
Previs, S
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Previs, S
;
Zhang, YT
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Zhang, YT
;
Bernal, D
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Bernal, D
;
Pons, S
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Pons, S
;
Shulman, GI
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Shulman, GI
;
Bonner-Weir, S
论文数: 0引用数: 0
h-index: 0
机构:Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA
Bonner-Weir, S
;
White, MF
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USAHarvard Univ, Sch Med, Howard Hughes Med Inst, Joslin Diabet Ctr,Dept Med, Boston, MA 02215 USA