Calorie restriction increases cell surface GLUT-4 in insulin-stimulated skeletal muscle

被引:75
作者
Dean, DJ
Brozinick, JT
Cushman, SW
Cartee, GD
机构
[1] Univ Wisconsin, Dept Kinesiol, Biodynam Lab, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Nutr Sci, Madison, WI 53706 USA
[3] NIDDKD, Expt Diabet Metab & Nutr Sect, Diabet Branch, Bethesda, MD 20892 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1998年 / 275卷 / 06期
关键词
food restriction; insulin signaling;
D O I
10.1152/ajpendo.1998.275.6.E957
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Reduced calorie intake [calorie restriction (CR); 60% of ad libitum (AL)] leads to enhanced glucose transport without altering total GLUT-4 glucose transporter abundance in skeletal muscle. Therefore, we tested the hypothesis that CR (20 days) alters the subcellular distribution of GLUT-I. Cell surface GLUT-4 content was higher in insulin-stimulated epitrochlearis muscles from CR vs. AL rats. The magnitude of this increase was similar to the CR-induced increase in glucose transport, and GLUT-4 activity (glucose transport rate divided by cell surface GLUT-4) was unaffected by diet. The CR effect was specific to the insulin-mediated pathway, as evidenced by the observations that basal glucose transport and cell surface GLUT-4 content, as well as hypoxia-stimulated glucose transport, were unchanged by diet. CR did not alter insulin's stimulation of insulin receptor substrate (IRS)-1-associated phosphatidylinositol 3-kinase (PI3K) activity. Muscle abundance of IRS-2 and p85 subunit of PI3K were unaltered by diet, but IRS-1 content was lower in CR vs. AL. These data demonstrate that, despite IRS-1-PI3K activity similar to AL, CR specifically increases insulin's activation of glucose transport by enhancing the steady-state proportion of GLUT-4 residing on the cell surface.
引用
收藏
页码:E957 / E964
页数:8
相关论文
共 46 条
[41]   Disruption of IRS-2 causes type 2 diabetes in mice [J].
Withers, DJ ;
Gutierrez, JS ;
Towery, H ;
Burks, DJ ;
Ren, JM ;
Previs, S ;
Zhang, YT ;
Bernal, D ;
Pons, S ;
Shulman, GI ;
Bonner-Weir, S ;
White, MF .
NATURE, 1998, 391 (6670) :900-904
[42]   Insulin signaling in human skeletal muscle - Time course and effect of exercise [J].
Wojtaszewski, JFP ;
Hansen, BF ;
Kiens, B ;
Richter, EA .
DIABETES, 1997, 46 (11) :1775-1781
[43]   THE EFFECTS OF WORTMANNIN ON RAT SKELETAL-MUSCLE - DISSOCIATION OF SIGNALING PATHWAYS FOR INSULIN-ACTIVATED AND CONTRACTION-ACTIVATED HEXOSE-TRANSPORT [J].
YEH, JI ;
GULVE, EA ;
RAMEH, L ;
BIRNBAUM, MJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (05) :2107-2111
[44]  
YOUNG DA, 1986, J BIOL CHEM, V261, P6049
[45]   GLUCOSE-TRANSPORT IS RATE LIMITING FOR SKELETAL-MUSCLE GLUCOSE-METABOLISM IN NORMAL AND STZ-INDUCED DIABETIC RATS [J].
ZIEL, FH ;
VENKATESAN, N ;
DAVIDSON, MB .
DIABETES, 1988, 37 (07) :885-890
[46]   High-fat feeding impairs insulin-stimulated GLUT4 recruitment via an early insulin-signaling defect [J].
Zierath, JB ;
Houseknecht, KL ;
Gnudi, L ;
Kahn, BB .
DIABETES, 1997, 46 (02) :215-223