An alternative renewable source of squalene for use in emulsion adjuvants

被引：29

作者：

Brito, Luis A. ^{[1
]}

Chan, Michelle ^{[1
]}

Baudner, Barbara ^{[2
]}

Gallorini, Simona ^{[2
]}

Santos, George ^{[1
]}

O'Hagan, Derek T. ^{[1
]}

Singh, Manmohan ^{[1
]}

机构：

[1] Novartis Vaccines & Diagnost, Cambridge, MA 02139 USA

[2] Novartis Vaccines & Diagnost, I-53100 Siena, Italy

来源：

VACCINE | 2011年 / 29卷 / 37期

关键词：

Adjuvant; Squalene; MF59; Influenza; Meningitis; FREUNDS-ADJUVANT; VACCINE ADJUVANT; INFECTION; IMMUNITY; MF59; FORMULATION; PROTECTION; DELIVERY; PROTEIN; BOVINE;

D O I：

10.1016/j.vaccine.2011.06.067

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Emulsions have been used to boost immunogenicity of antigens since the discovery of complete Freunds adjuvant. Optimization to reduce reactogenicity of emulsion adjuvants lead to the development of oil in water emulsions based on squalene. MF59 is an oil-in-water emulsion that is a component of an approved influenza product in Europe. Currently MF59 is manufactured from squalene derived from an animal source. Recently a high purity plant-derived squalene source has become available at an appropriate purity for a vaccine adjuvant. The purpose of this study was to evaluate and compare animal-derived squalene and plant-derived squalene for equivalency. Nanoemulsions were prepared and analyzed for size and viscosity prepared from each source. The two emulsions were administered in two separate animal studies, one focusing on Neisseria meningitidis B, and one focusing on influenza. Readouts were ELISA titers for each antigen and serum bactericidal activity for N. meningitidis B, and hemagglutinin inhibition for influenza to see the functionality of the antibodies produced. Results indicate that there are no differences between the antibodies elicited after immunization from an emulsion made with oil derived from either an animal or plant-source. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：6262 / 6268

页数：7

共 32 条

[1]

Neisseria meningitidis group B correlates of protection and assay standardization -: International meeting report Emory University, Atlanta, Georgia, United States, 16-17 march 2005 [J].

Borrow, R. ;

Carlone, G. M. ;

Rosenstein, N. ;

Plikaytis, B. ;

Blake, M. ;

Feavers, I. ;

Martin, D. ;

Zollinger, W. ;

Robbins, J. ;

Aaberge, I. ;

Granoff, D. M. ;

Miller, E. ;

van Alphen, L. ;

Poolman, J. ;

Rappuoli, R. ;

Danzig, L. ;

Hackell, J. ;

Danve, B. ;

Caulfield, M. ;

Lambert, S. ;

Stephens, D. .

VACCINE, 2006, 24 (24) :5093-5107

[2]

ADJUVANT FORMULATION FOR USE IN VACCINES TO ELICIT BOTH CELL-MEDIATED AND HUMORAL IMMUNITY [J].

BYARS, NE ;

ALLISON, AC .

VACCINE, 1987, 5 (03) :223-228

[3]

Vaccines with the MF59 adjuvant do not stimulate antibody responses against squalene [J].

Del Giudice, Giuseppe ;

Fragapane, Elena ;

Bugarini, Roberto ;

Hora, Maninder ;

Henriksson, Thomas ;

Palla, Emanuela ;

O'Hagan, Derek ;

Donnelly, John ;

Rappuoli, Rino ;

Podda, Audino .

CLINICAL AND VACCINE IMMUNOLOGY, 2006, 13 (09) :1010-1013

[4]

Edelman R, 2004, ADJUVANTS FUTURE

[5]

EVANS AS, 1975, YALE J BIOL MED, V48, P201

[6]

Monitoring the effects of component structure and source on formulation stability and adjuvant activity of oil-in-water emulsions [J].

Fox, Christopher B. ;

Anderson, Ryan C. ;

Dutill, Timothy S. ;

Goto, Yasuyuki ;

Reed, Steven G. ;

Vedvick, Thomas S. .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2008, 65 (01) :98-105

[7]

Squalene Emulsions for Parenteral Vaccine and Drug Delivery [J].

Fox, Christopher B. .

MOLECULES, 2009, 14 (09) :3286-3312

[8]

FREUND J, 1956, Bibl Tuberc, P130

[9]

Sensitization and antibody formation with increased resistance to tuberculous infection induced by heat killed tubercle bacilli [J].

Freund, J ;

Opie, EL .

JOURNAL OF EXPERIMENTAL MEDICINE, 1938, 68 (02) :273-298

[10]

First-in-human clinical trials with vaccines-what regulators want [J].

Goetz, Karen B. ;

Pfleiderer, Michael ;

Schneider, Christian K. .

NATURE BIOTECHNOLOGY, 2010, 28 (09) :910-916

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