Imaging mass spectrometry for lipidomics

被引：147

作者：

Goto-Inoue, Naoko ^{[1
]}

Hayasaka, Takahiro ^{[1
]}

Zaima, Nobuhiro ^{[2
]}

Setou, Mitsutoshi ^{[1
]}

机构：

[1] Hamamatsu Univ Sch Med, Dept Mol Anat, Mol Imaging Frontier Res Ctr, Higashi Ku, Hamamatsu, Shizuoka 4313192, Japan

[2] Kinki Univ, Dept Appl Biol Chem, Grad Sch Agr, Higashiosaka, Osaka 577, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2011年 / 1811卷 / 11期

基金：

日本科学技术振兴机构;

关键词：

Lipidomics; Imaging mass spectrometry; Matrix-assisted laser desorption/ionization; DESORPTION ELECTROSPRAY-IONIZATION; THIN-LAYER-CHROMATOGRAPHY; PRINCIPAL COMPONENT ANALYSIS; DIRECT TISSUE-ANALYSIS; LEMLI-OPITZ-SYNDROME; ION MOBILITY-TOFMS; DOCOSAHEXAENOIC ACID; MOUSE-BRAIN; FATTY-ACIDS; RETINAL PHOTORECEPTORS;

D O I：

10.1016/j.bbalip.2011.03.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-MS technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. This technique can reveal the distribution of hundreds of ion signals in a single measurement and also helps in understanding the cellular profile of the biological system. MALDI-IMS has already revealed the characteristic distribution of several kinds of lipids in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields, especially in lipidomics. In this review, we describe the methodology and applications of MALDI-IMS to biological samples. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrometry. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：961 / 969

页数：9

共 99 条

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