PCR and restriction endonuclease assay for detection of a novel mutation associated with sulfonamide resistance in Neisseri meningitidis

被引：6

作者：

Bennett, DE

Cafferkey, MT

机构：

[1] Childrens Univ Hosp, Epidemiol & Mol Biol Unit, Dublin 1, Ireland

[2] Royal Coll Surgeons Ireland, Dept Clin Microbiol, Dublin 2, Ireland

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 10期

关键词：

D O I：

10.1128/AAC.47.10.3336-3338.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We identified a previously undocumented mutation in the dihydropteroate synthase (folP) gene associated with Neisseria meningitidis sulfonamide resistance. A PCR-based assay to detect this mutation, which is 100% predictive of sulfonamide resistance, was developed.

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页码：3336 / 3338

页数：3

相关论文

共 17 条

[11] Mutations and horizontal transmission have contributed to sulfonamide resistance in Streptococcus pyogenes [J].

Jönsson, M ;

Ström, K ;

Swedberg, G .

MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 2003, 9 (02) :147-153

[12] PCR AMPLICON RESTRICTION-ENDONUCLEASE ANALYSIS OF THE CHROMOSOMAL DHPS GENE OF NEISSERIA-MENINGITIDIS - A METHOD, FOR STUDYING SPREAD OF THE DISEASE-CAUSING STRAIN IN CONTACTS OF PATIENTS WITH MENINGOCOCCAL DISEASE [J].

KRISTIANSEN, BE ;

FERMER, C ;

JENKINS, A ;

ASK, E ;

SWEDBERG, G ;

SKOLD, O .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1174-1179

[13]

PELTOLA H, 1983, REV INFECT DIS, V5, P71

[14] Additive effects of a two-amino-acid insertion and a single-amino-acid substitution in dihydropteroate synthase for the development of sulphonamide-resistant Neisseria meningitidis [J].

Qvarnström, Y ;

Swedberg, G .

MICROBIOLOGY-SGM, 2000, 146 :1151-1156

[15]

RADSTROM P, 1992, J BACTERIOL, V174, P6386

[16] Sulfonamide resistance:: mechanisms and trends [J].

Sköld, O .

DRUG RESISTANCE UPDATES, 2000, 3 (03) :155-160

[17] SULFONAMIDE RESISTANCE IN NEISSERIA-MENINGITIDIS STRAINS LIBERATING VARIOUS AMOUNTS OF FREE ENDOTOXIN [J].

SOLBERG, O ;

ANDERSEN, BM .

SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 1983, 15 (02) :149-151