Photoaffinity labeling as an approach to study supramolecular nucleoprotein complexes

被引：40

作者：

Knorre, DG ^{[1
]}

Godovikova, TS ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Bioorgan Chem, Siberian Div, Novosibirsk 630090, Russia

来源：

FEBS LETTERS | 1998年 / 433卷 / 1-2期

关键词：

photoaffinity modification; nucleoprotein; photoreactive nucleic acid; arylazide; photomodified amino acid residue;

D O I：

10.1016/S0014-5793(98)00860-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The modern approaches for studying the detailed structure of nucleoprotein complexes involved in replication and transcription, based on the use of nucleic acids with photoreactive groups incorporated into definite positions of polynucleotide chain, are considered. Methods of preparation of photoreactive nucleic acids of this type are presented, Their use for positioning of RNA polymerase III and transcription factors as well as of the main participants of the replication machinery at the respective templates is described, A survey of the data concerning the amino acid residues modified in the course of photoaffinity labeling of proteins is also presented and some complications are discussed. (C) 1998 Federation of European Biochemical Societies.

引用

页码：9 / 14

页数：6

共 50 条

[31] Photocross-linking of nucleic acids to associated proteins [J].

Meisenheimer, KM ;

Koch, TH .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1997, 32 (02) :101-140

[32] DNA polymerase photoprobe 2-[(4-azidophenacyl)thio]-2'-deoxyadenosine 5'-triphosphate labels an Escherichia coli DNA polymerase I Klenow fragment substrate binding site [J].

Moore, BM ;

Jalluri, RK ;

Doughty, MB .

BIOCHEMISTRY, 1996, 35 (36) :11642-11651

[33]

MYSZKA DG, 1991, J BIOL CHEM, V266, P4789

[34] IDENTIFICATION OF AN ACTIVE-SITE PEPTIDE OF SKELETAL MYOSIN AFTER PHOTOAFFINITY-LABELING WITH N-(4-AZIDO-2-NITROPHENYL)-2-AMINOETHYL DIPHOSPHATE [J].

OKAMOTO, Y ;

YOUNT, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (06) :1575-1579

[35]

PARK CS, 1982, J BIOL CHEM, V257, P6590

[36] ESCHERICHIA-COLI DNA-POLYMERASE-III HOLOENZYME SUBUNIT-ALPHA, SUBUNIT-BETA, AND SUBUNIT-GAMMA DIRECTLY CONTACT THE PRIMER-TEMPLATE [J].

REEMS, JA ;

WOOD, S ;

MCHENRY, CS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (10) :5606-5613

[37]

RINGHEIM GE, 1988, J BIOL CHEM, V263, P18247

[38]

RINGHEIM GE, 1990, J BIOL CHEM, V265, P19472

[39] DIFFERENTIAL-T CELL-RECEPTOR PHOTOAFFINITY-LABELING AMONG H-2KD RESTRICTED CYTOTOXIC T-LYMPHOCYTE CLONES SPECIFIC FOR A PHOTOREACTIVE PEPTIDE DERIVATIVE - LABELING OF THE ALPHA-CHAIN CORRELATES WITH J-ALPHA SEGMENT USAGE [J].

ROMERO, P ;

CASANOVA, JL ;

CEROTTINI, JC ;

MARYANSKI, JL ;

LUESCHER, IF .

JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (05) :1247-1256

[40] Specific photoaffinity labeling of Tyr-49 on the light chain in the steroid-combining site of a mouse monoclonal anti-estradiol antibody using two epimeric 6 alpha- and 6 beta-(5-azido-2-nitrobenzoyl)amidoestradiol photoreagents [J].

Rousselot, P ;

Mappus, E ;

Blachere, T ;

deRavel, MR ;

Grenot, C ;

Tonnelle, C ;

Cuilleron, CY .

BIOCHEMISTRY, 1997, 36 (25) :7860-7868

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