Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine

被引：48

作者：

Schofield, DJ ^{[1
]}

Purcell, RH ^{[1
]}

Nguyen, HT ^{[1
]}

Emerson, SU ^{[1
]}

机构：

[1] NIAID, Lab Infect Dis, Hepatitis Viruses & Mol Hepatitis Sect, NIH, Bethesda, MD 20892 USA

来源：

VACCINE | 2003年 / 22卷 / 02期

关键词：

Hepatitis E virus; phage display; neutralization site;

D O I：

10.1016/j.vaccine.2003.07.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to obtain monoclonal antibodies that might have prophylactic applications and to understand better the immune response to hepatitis E virus (HEV), we used phage display to isolate chimpanzee antibodies to HEV. The panning antigen was an two open reading frame (ORF2) recombinant protein that elicits a broadly protective immune response in vaccinated monkeys. Two major antigenic sites were identified on the ORF2 protein: one site was not accessible on the surface of infectious virions but the other site was accessible to antibodies and was recognized specifically by antibodies that neutralize the virus. Published by Elsevier Ltd.

引用

页码：257 / 267

页数：11

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