RFLP- and physical mapping of resistance gene homologues in rice (O-sativa) and barley (H-vulgare)

被引：58

作者：

Leister, D

Kurth, J

Laurie, DA

Yano, M

Sasaki, T

Graner, A

Schulze-Lefert, P

机构：

[1] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

[2] STAFF Inst, Rice Genome Res Program, Ibaraki, Osaka 305, Japan

[3] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 1999年 / 98卷 / 3-4期

关键词：

resistance gene; RFLP; YAC contig; fragmentation; gene cluster;

D O I：

10.1007/s001220051099

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The deduced peptide sequences of 25 gene fragments of NBS-LRR resistance (R) gene homologues from rice and barley and of characterized R genes were compared, revealing a string of six conserved motifs. Mapping of the R-gene candidates in rice showed linkage to genes conferring race-specific resistance to rice blast (Pi-k, Pi-f and Pi-1) and bacterial blight disease (Xa-1, Xa-3 and Xa-4), in barley to powdery mildew (Mla) and the rust fungus (Rpg1). In rice four mixed clusters were detected, each harboring at least two highly dissimilar NBS-LRR genes. A YAC-contig was established for one of these mixed clusters. YAC fragmentation experiments revealed the presence of at least five NBS-LRR genes within 200 kb in head-to-tail orientation.

引用

页码：509 / 520

页数：12

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