Porcine alveolar macrophages: poor accessory or effective suppressor cells for T-lymphocytes

被引:28
作者
Basta, S [1 ]
Carrasco, CP [1 ]
Knoetig, SM [1 ]
Rigden, RC [1 ]
Gerber, H [1 ]
Summerfield, A [1 ]
McCullough, KC [1 ]
机构
[1] Inst Virol & Immunoprophylaxis, CH-3147 Mittelhausern, Switzerland
关键词
alveolar macrophages; accessory function; IL-1;
D O I
10.1016/S0165-2427(00)00237-3
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Porcine Alv-Mempty set from bronchoalveolar lavages were tested for their function in an in vitro foot-and-mouth disease virus (FMDV)-specific lymphoproliferative recall response. The Alv-Mempty set were seen to be poor accessory cells when compared with peripheral blood monocytes. This poor capacity was evident despite an efficient expression of SLA-DR region antigens, and other costimulatory adhesion molecules. It was noted that Alv-Mempty set secrete relatively little interleukin 1 (IL-1 beta), with or without LPS induction, even though mRNA for the cytokine could be detected. In contrast, blood monocytes with their effective accessory activity were potent secretors of IL-1. Although this IL-1 beta would be important with respect to the accessory capacity of monocytic cells, it was noted that the absence of bioactive IL-1 from the Alv-Mempty set cultures was not solely responsible for their poor accessory function. In fact, the Alv-Mempty set produced factors which not only inhibited IL-1 bioactivity, but were also responsible for a clear suppression of lymphoproliferation. This suppressor activity was dependent on the type of monocytic cell present in the culture. being more prominent when "scavenger" phagocytes were present. Thus, the major role of Alv-Mempty set is not as an accessory cell akin to monocytes, but as both a scavenger cell, related to Mempty set derived from monocytes in the absence of inflammatory signals, and an immunoregulatory cell. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:177 / 190
页数:14
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