Characterizing the chromosomes of the platypus (Ornithorhynchus anatinus)

被引:13
作者
McMillan, Daniel [1 ]
Miethke, Pat [1 ]
Alsop, Amber E. [1 ]
Rens, Willem [2 ]
O'Brien, Patricia [2 ]
Trifonov, Vladimir [2 ]
Veyrunes, Frederic [1 ,2 ]
Schatzkamer, Kyriena [3 ]
Kremitzki, Colin L. [3 ]
Graves, Tina [3 ]
Warren, Wesley [3 ]
Grutzner, Frank [4 ]
Ferguson-Smith, Malcolm A. [2 ]
Graves, Jennifer A. Marshall [1 ]
机构
[1] Australian Natl Univ, Res Sch Biol Sci, Comparat Genom Grp, Canberra, ACT 2601, Australia
[2] Univ Cambridge, Dept Vet Med, Ctr Vet Med, Mol Cytogenet Lab, Cambridge CB3 0ES, England
[3] Washington Univ, Sch Med, Genome Sequencing Ctr, St Louis, MO 63108 USA
[4] Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA 5005, Australia
关键词
chromosome painting; karyology; Ornithorhynchus anatinus; platypus; sex chromosomes;
D O I
10.1007/s10577-007-1186-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Like the unique platypus itself, the platypus genome is extraordinary because of its complex sex chromosome system, and is controversial because of difficulties in identification of small autosomes and sex chromosomes. A 6-fold shotgun sequence of the platypus genome is now available and is being assembled with the help of physical mapping. It is therefore essential to characterize the chromosomes and resolve the ambiguities and inconsistencies in identifying autosomes and sex chromosomes. We have used chromosome paints and DAPI banding to identify and classify pairs of autosomes and sex chromosomes. We have established an agreed nomenclature and identified anchor BAC clones for each chromosome that will ensure unambiguous gene localizations.
引用
收藏
页码:961 / 974
页数:14
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