Whole-Genome Characterization of Human and Simian Immunodeficiency Virus Intrahost Diversity by Ultradeep Pyrosequencing

被引：52

作者：

Bimber, Benjamin N. ^{[1
]}

Dudley, Dawn M. ^{[2
]}

Lauck, Michael ^{[2
]}

Becker, Ericka A. ^{[1
]}

Chin, Emily N. ^{[2
]}

Lank, Simon M. ^{[1
]}

Grunenwald, Haiying L. ^{[5
]}

Caruccio, Nicholas C. ^{[5
]}

Maffitt, Mark ^{[5
]}

Wilson, Nancy A. ^{[1
]}

Reed, Jason S. ^{[1
]}

Sosman, James M. ^{[6
]}

Tarosso, Leandro F. ^{[4
]}

Sanabani, Sabri ^{[4
]}

Kallas, Esper G. ^{[4
]}

Hughes, Austin L. ^{[3
]}

O'Connor, David H. ^{[1
,2
]}

机构：

[1] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53706 USA

[3] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA

[4] Univ Sao Paulo, Div Clin Immunol & Allergy, Sao Paulo, Brazil

[5] Epictr Biotechnol, Madison, WI 53713 USA

[6] Univ Wisconsin, Carbone Canc Ctr, Madison, WI 53705 USA

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 22期

基金：

美国国家卫生研究院; 巴西圣保罗研究基金会;

关键词：

CYTOTOXIC T-LYMPHOCYTES; ESCAPE; VARIANTS; HIV-1;

D O I：

10.1128/JVI.01378-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rapid evolution and high intrahost sequence diversity are hallmarks of human and simian immunodeficiency virus (HIV/SIV) infection. Minor viral variants have important implications for drug resistance, receptor tropism, and immune evasion. Here, we used ultradeep pyrosequencing to sequence complete HIV/SIV genomes, detecting variants present at a frequency as low as 1%. This approach provides a more complete characterization of the viral population than is possible with conventional methods, revealing low-level drug resistance and detecting previously hidden changes in the viral population. While this work applies pyrosequencing to immunodeficiency viruses, this approach could be applied to virtually any viral pathogen.

引用

页码：12087 / 12092

页数：6

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