Endonuclease IV homolog from Dictyostelium discoideum:: sequencing and functional expression in AP endonuclease-deficient Escherichia coli

被引：1

作者：

Tsuji, A ^{[1
]}

Kodaira, K ^{[1
]}

Inoue, M ^{[1
]}

Yasukawa, H ^{[1
]}

机构：

[1] Toyama Univ, Fac Engn, Div Bioengn, Toyama 9308555, Japan

来源：

MUTATION RESEARCH-DNA REPAIR | 2001年 / 486卷 / 01期

关键词：

Dictyostelium; AP endonuclease; DNA repair;

D O I：

10.1016/S0921-8777(01)00074-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We sequenced a gene encoding AP endonuclease DdAPN in Dictyostelium discoideum. The sequence predicts a protein of 542 amino acids, showing high homology to Escherichia coli Endonuclease TV (Endo TV). There is 45% identity to Endo TV using the C-terminal 282 amino acids of the Dictyostelium protein. The DdAPN conserves nine residues for the metal-binding identified in Endo IV. The truncated DdAPN protein containing these sites partially complemented E. coli RPC501 (xth(-), nfo(-)). (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：53 / 57

页数：5

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