Enantiomeric separation of ketoconazole and terconazole antifungals by electrokinetic chromatography: Rapid quantitative analysis of ketoconazole in pharmaceutical formulations

被引：32

作者：

Castro-Puyana, M ^{[1
]}

Crego, AL ^{[1
]}

Marina, ML ^{[1
]}

机构：

[1] Univ Alcala de Henares, Fac Quim, Dept Quim Analit, E-28871 Alcala De Henares, Spain

来源：

ELECTROPHORESIS | 2005年 / 26卷 / 20期

关键词：

antifungals; chiral separation; cyclodextrins; electrokinetic chromatography; ketoconazole; terconazole;

D O I：

10.1002/elps.200500100

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

EKC using a neutral CID as chiral selector was applied in this work to the development of a method enabling the enantiomeric separation of ketoconazole and terconazole antifungals. The influence of different experimental conditions such as temperature, CID concentration, pH, and nature and concentration of the buffer on the enantiomeric resolution of the compounds studied was investigated. The use of 10 mm heptakis(2,3,6-tri-O-methyl)-beta-CD in a 100 mm phosphate buffer (pH 3.5) with a temperature of 15 degrees C allowed the separation of the enantiomers of ketoconazole and terconazole with high resolution (R-s > 2.0). The rapid separation of ketoconazole enantiomers with an analysis time less than 3 min was carried out after fitting some experimental parameters. The developed method was applied to the determination of ketoconazole in different pharmaceutical formulations.

引用

页码：3960 / 3968

页数：9

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