Tau protein is involved in morphological plasticity in hippocampal neurons in response to BDNF

被引：64

作者：

Chen, Qian ^{[1
]}

Zhou, Zhou ^{[1
]}

Zhang, Lei ^{[1
]}

Wang, Yuan ^{[1
]}

Zhang, Yan-wen ^{[1
]}

Zhong, Min ^{[1
]}

Xu, Shang-cheng ^{[1
]}

Chen, Chun-hai ^{[1
]}

Li, Li ^{[1
]}

Yu, Zheng-ping ^{[1
]}

机构：

[1] Third Mil Med Univ, Dept Occupat Hlth, Key Lab Med Protect Electromagnet Radiat, Minist Educ, Chongqing 400038, Peoples R China

来源：

NEUROCHEMISTRY INTERNATIONAL | 2012年 / 60卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Tau; BDNF; Hippocampus; Morphological plasticity; MICROTUBULE-ASSOCIATED PROTEINS; ALZHEIMERS-DISEASE; SYNAPTIC PLASTICITY; NEUROTROPHIC FACTOR; NEURODEGENERATIVE DISORDERS; ANTISENSE OLIGONUCLEOTIDES; SPINE MORPHOLOGY; LIVING CELLS; PHOSPHORYLATION; DYNAMICS;

D O I：

10.1016/j.neuint.2011.12.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tau protein, a microtubule-associated protein involved in a number of neurological disorders such as Alzheimer's disease (AD), may undergo modifications under both physiological and pathological conditions. However, the signaling pathways that couple tau protein to neuronal physiology such as synaptic plasticity have not yet been elucidated. Here we report that tau protein is involved in morphological plasticity in response to brain derived neurotrophic factor (BDNF). Stimulation of the cultured rat hippocampal neurons with BDNF resulted in increased tau protein expression, as detected by Western blotting. Furthermore, tau protein accumulated in the distal region of the neurite when treated with taxol or taxol plus BDNF. The increased tau protein also protected neurons against nocodazole-induced dendrite loss. Moreover, BDNF promoted spine growth as well as tau protein over-expression. Knockdown of tau protein using specific short-hairpin RNA (shRNA) significantly decreased the spine density. And BDNF could not increase the spine density of tau-knockdown neurons. These results highlight a possible role for tau protein in the dynamic rearrangement of cytoskeletal fibers vital for BDNF-induced synaptic plasticity. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：233 / 242

页数：10

共 46 条

[1] Hyperphosphorylation induces self-assembly of τ into tangles of paired helical filaments/straight filaments [J].