Acid sensing by the Drosophila olfactory system

被引:260
作者
Ai, Minrong [1 ]
Min, Soohong [1 ]
Grosjean, Yael [2 ]
Leblanc, Charlotte [1 ]
Bell, Rati [2 ]
Benton, Richard [2 ]
Suh, Greg S. B. [1 ]
机构
[1] NYU, Sch Med, Dept Cell Biol, Skirball Inst Biomol Med,Mol Neurobiol Program, New York, NY 10016 USA
[2] Univ Lausanne, Fac Biol & Med, Ctr Integrat Genom, CH-1015 Lausanne, Switzerland
基金
欧洲研究理事会; 瑞士国家科学基金会;
关键词
CARBON-DIOXIDE; CHEMOSENSORY RECEPTORS; TARGETED EXPRESSION; AVOIDANCE; NEURONS; TRANSMISSION; BEHAVIOR; REVEALS; MINOS; MAP;
D O I
10.1038/nature09537
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The odour of acids has a distinct quality that is perceived as sharp, pungent and often irritating(1). How acidity is sensed and translated into an appropriate behavioural response is poorly understood. Here we describe a functionally segregated population of olfactory sensory neurons in the fruitfly, Drosophila melanogaster, that are highly selective for acidity. These olfactory sensory neurons express IR64a, a member of the recently identified ionotropic receptor (IR) family of putative olfactory receptors(2). In vivo calcium imaging showed that IR64a+ neurons projecting to the DC4 glomerulus in the antennal lobe are specifically activated by acids. Flies in which the function of IR64a+ neurons or the IR64a gene is disrupted had defects in acid-evoked physiological and behavioural responses, but their responses to non-acidic odorants remained unaffected. Furthermore, artificial stimulation of IR64a+ neurons elicited avoidance responses. Taken together, these results identify cellular and molecular substrates for acid detection in the Drosophila olfactory system and support a labelled-line mode of acidity coding at the periphery.
引用
收藏
页码:691 / U112
页数:6
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