Rapid CE microbial assays for consumer products that contain active bacteria

被引:84
作者
Armstrong, DW [1 ]
Schneiderheinze, JM [1 ]
Kullman, JP [1 ]
He, LF [1 ]
机构
[1] Iowa State Univ, Dept Chem, Ames, IA 50011 USA
关键词
capillary electrophoresis; cell viability; L; acidophilus; B; infantis;
D O I
10.1111/j.1574-6968.2001.tb09442.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Recent advances in high efficiency separation methods of bacteria allow their rapid identification and quantitation in some cases. A specific capillary electrophoresis (CE) technique is used to identify and quantitate Lactobacillus acidophilus ill both pill and syrup health products as well as Bifidobacterium infantis in a powdered formula supplement. Cell viability can be evaluated as well. In some cases, both the living and dead bacterial cells as well as the molecular excipients can be evaluated in a single run. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:33 / 37
页数:5
相关论文
共 9 条
[1]  
[Anonymous], 1978, Intestinal Bacteria and Health
[2]   Separating microbes in the manner of molecules. 1. Capillary electrokinetic approaches [J].
Armstrong, DW ;
Schulte, G ;
Schneiderheinze, JM ;
Westenberg, DJ .
ANALYTICAL CHEMISTRY, 1999, 71 (24) :5465-5469
[3]  
EHRLICH HL, 1985, BACTERIA NATURE, V1, P189
[4]   Management of lactose maldigestion by consuming milk containing lactobacilli [J].
Lin, MY ;
Yen, CL ;
Chen, SH .
DIGESTIVE DISEASES AND SCIENCES, 1998, 43 (01) :133-137
[5]   STRAINS AND SPECIES OF LACTIC-ACID BACTERIA IN FERMENTED MILKS (YOGURTS) - EFFECT ON INVIVO LACTOSE DIGESTION [J].
MARTINI, MC ;
LEREBOURS, EC ;
LIN, WJ ;
HARLANDER, SK ;
BERRADA, NM ;
ANTOINE, JM ;
SAVAIANO, DA .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1991, 54 (06) :1041-1046
[6]   Improvement of lactose digestion by humans following ingestion of unfermented acidophilus milk: Influence of bile sensitivity, lactose transport, and acid tolerance of Lactobacillus acidophilus [J].
Mustapha, A ;
Jiang, TA ;
Savaiano, DA .
JOURNAL OF DAIRY SCIENCE, 1997, 80 (08) :1537-1545
[7]   High efficiency separation of microbial aggregates using capillary electrophoresis [J].
Schneiderheinze, JM ;
Armstrong, DW ;
Schulte, G ;
Westenberg, DJ .
FEMS MICROBIOLOGY LETTERS, 2000, 189 (01) :39-44
[8]  
Suarez FL, 1995, ALIMENT PHARM THER, V9, P589, DOI 10.1111/j.1365-2036.1995.tb00427.x
[9]  
Vesa TH, 1996, EUR J CLIN NUTR, V50, P730