Cross-adaptation between olfactory responses induced by two subgroups of odorant molecules

被引:19
作者
Takeuchi, H [1 ]
Imanaka, Y
Hirono, J
Kurahashi, T
机构
[1] Osaka Univ, Dept Frontier Biosci, Toyonaka, Osaka 5608531, Japan
[2] Osaka Univ, Dept Biophys Engn, Toyonaka, Osaka 5608531, Japan
[3] Natl Inst Adv Ind Sci & Technol, Tissue Engn Res Ctr, Amagasaki, Hyogo 6610974, Japan
关键词
InsP(3); cAMP; olfactory receptor cell; signal transduction; adaptation;
D O I
10.1085/jgp.200308867
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
It has long been believed that vertebrate olfactory signal transduction is mediated by independent multiple pathways (using cAMP and InsP(3) as second messengers). However, the dual presence of parallel pathways in the olfactory receptor cell is still controversial, mainly because of the lack of information regarding the single-cell response induced by odorants that have been shown to produce InsP(3) exclusively (but not cAMP) in the olfactory cilia. In this study, we recorded activities of transduction channels of single olfactory receptor cells to InsP(3)-producing odorants. When the membrane potential was held at -54 mV, application of InsP(3)-producing odorants to the ciliary region caused an inward current. The reversal potential was 0 +/- 7 mV (mean +/- SD, n = 10). Actually, InsP(3)-producing odorants generated responses in a smaller fraction of cells (lilial, 3.4%; lyral, 1.7%) than the cAMP-producing odorant (cineole, 26%). But, fundamental properties of responses were surprisingly homologous; namely, spatial distribution of the sensitivity, waveforms, IN relation, and reversal potential, dose dependence, time integration of stimulus period, adaptation, and recovery. By applying both types of odorants alternatively to the same cell, furthermore, we observed cells to exhibit symmetrical cross-adaptation. It seems likely that even with odorants with different modalities adaptation occurs completely depending on the amount of current flow. The data will also provide evidence showing that olfactory response generation and adaptation are regulated by a uniform mechanism for a wide variety of odorants.
引用
收藏
页码:255 / 264
页数:10
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