Detergent resistance as a tool in membrane research

被引：219

作者：

Lingwood, Daniel ^{[1
]}

Simons, Kai ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 09期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1038/nprot.2007.294

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The biological membrane is a complicated matrix wherein different lipid environments are thought to exist. The more ordered or raft environment has been perceived biochemically accessible via its relative resistance to detergent. This paper outlines the protocols developed in our laboratory for the analysis of such detergent-resistant membranes (DRMs). We stress the fact that DRMs are artifactual in nature and should not be equivocated to lipid rafts, their usefulness being limited to assigning raft-association potential most convincingly when changes in DRM composition are induced by biochemically/physiologically relevant events. These protocols are completed in 1-2 d.

引用

页码：2159 / 2165

页数：7

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