Fingerprint-efficacy study of artificial Calculus bovis in quality control of Chinese materia medica

被引：52

作者：

Kong, Weijun ^{[1
,2
]}

Wang, Jiabo ^{[1
]}

Zang, Qingce ^{[1
,3
]}

Xing, Xiaoyan ^{[1
,2
]}

Zhao, Yanling ^{[1
]}

Liu, Wei ^{[3
]}

Jin, Cheng ^{[1
]}

Li, Zulun ^{[2
]}

Xiao, Xiaohe ^{[1
]}

机构：

[1] 302 Mil Hosp China, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China

[2] Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 611137, Peoples R China

[3] Jiangxi Univ Tradit Chinese Med, Coll Pharm, Nanchang 330004, Peoples R China

来源：

FOOD CHEMISTRY | 2011年 / 127卷 / 03期

基金：

美国国家科学基金会;

关键词：

Artificial Calculus bovis; Fingerprint-efficacy; Chemical fingerprint; Antibacterial effect; Quality control; PRINCIPAL COMPONENT ANALYSIS; BILE-ACID DERIVATIVES;

D O I：

10.1016/j.foodchem.2011.01.095

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

For quality control of Chinese materia medica (CMM), an attempt on fingerprint-efficacy study of artificial Calculus bovis was developed in this work. Chemical fingerprints of artificial C. bovis samples from ten different sources were determined by UPLC-ELSD and investigated by similarity analysis and hierarchical clustering analysis. Antibacterial effects of these samples on Escherichia coli growth were measured using microcalorimetry. The fingerprint-efficacy relationship of chemical fingerprint and antibacterial effect of artificial C. bovis were established by multi-linear regression analysis. Our results showed that the sources and places of production of artificial C. bovis had some important influence on the chemical fingerprints and antibacterial effects of this CMM. These artificial C. bovis could be grouped into four clusters according to their chemical fingerprints and antibacterial effects. Compounds cholic acid, taurocholate sodium, hyodeoxycholic acid and one unknown compound might be the major effective components for quality control of this CMM. Fingerprint-efficacy study provided a powerful way and some insight for the quality control of artificial C. bovis and other CCMs. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

引用

页码：1342 / 1347

页数：6

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