Sensitive and rapid detection of Aeromonas caviae in stool samples by loop-mediated isothermal amplification

被引：15

作者：

Wei, Xiao Na ^{[1
]}

Zheng, Ze Jun ^{[1
]}

Zhang, Li Huan ^{[2
,3
]}

Qu, Fen

Huang, Xitai ^{[1
]}

机构：

[1] Nankai Univ, Coll Life Sci, Dept Biochem & Mol Biol, Tianjin 300071, Peoples R China

[2] Tianjin Entry Exit Inspect & Quarantine Bur, Tianjin 300457, Peoples R China

[3] Peoples Liberat Army, Ctr Clin Lab, Hosp 302, Beijing 100039, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 60卷 / 01期

关键词：

Aeromonas caviae; loop-mediated isothermal amplification (LAMP); ITS;

D O I：

10.1016/j.diagmicrobio.2007.07.021

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In this study, a loop-mediated isothermal amplification assay targeting the 16S-23S intergenic spacer regions (internal transcribed spacer) of Aeromonas caviae was developed. Eighteen reference strains and 109 clinical samples were analyzed. The results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods. (c) 2008 Published by Elsevier Inc.

引用

页码：113 / 116

页数：4

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