The parameters of guard cell calcium oscillation encodes stomatal oscillation and closure in Vicia faba

Oscillations in cytosolic calcium ([Ca2+](cyt)) are an important component of Ca2+-based signal transduction cascades and have been proposed as necessary for stomatal closure. The research about the roles of individual [Ca2+](cyt) oscillations parameters in regulating stomatal closure have been touched, but there still have many problems remaining largely unknown. We systematically vary the [Ca2+](cyt) oscillation parameters in Vicia faba L. guard cells to investigate their roles in stomatal movements. [Ca2+](cyt) oscillations could induce synchronous stomatal oscillations and regulate the short-term stomatal closure during the oscillation. The direct effect of [Ca2+](cyt) on the activity of aquaporin may be the main cause of the stomatal oscillation induced by [Ca2+](cyt) oscillations. Stomatal dynamics indicate the long-term steady-state stomatal closure can be apparently elicited by optimal [Ca2+](cyt) oscillation transient numbers and periods. The stomata in different growing phases have the different responses to the same [Ca2+]c(yt) oscillation parameters. (C) 2003 Elsevier Ireland Ltd. All rights reserved.