Isolation of renal proximal tubular brush-border membranes

被引：105

作者：

Biber, Juerg ^{[1
]}

Stieger, Bruno ^{[2
]}

Stange, Gerti ^{[1
]}

Murer, Heini ^{[1
]}

机构：

[1] Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland

[2] Univ Zurich Hosp, Dept Med, Div Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 06期

关键词：

D O I：

10.1038/nprot.2007.156

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.

引用

页码：1356 / 1359

页数：4

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