Use of the tna operon as a new molecular target for Escherichia coli detection

被引：7

作者：

Bernasconi, Camilla

Volponi, Giorgio

Picozzi, Claudia

Foschino, Roberto

机构：

[1] Inst Hlth & Consumer Protect, Joint Res Ctr, Ispra, VA, Italy

[2] Univ Milan, DiSTAM, Milan, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 19期

关键词：

D O I：

10.1128/AEM.00606-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon.

引用

页码：6321 / 6325

页数：5

共 20 条

[1]

[Anonymous], 2006, Official Journal of the European Union L, V64, P37

[2]

[Anonymous], 2016, 16140 ISO

[3]

DETECTION OF ESCHERICHIA-COLI AND SHIGELLA SPP IN WATER BY USING THE POLYMERASE CHAIN-REACTION AND GENE PROBES FOR UID [J].

BEJ, AK ;

DICESARE, JL ;

HAFF, L ;

ATLAS, RM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (04) :1013-1017

[4]

Molecular strategies for the detection, identification, and differentiation between enteroinvasive Escherichia coli and Shigella spp. [J].

Bin Kingombe, CI ;

Cerqueira-Campos, ML ;

Farber, JM .

JOURNAL OF FOOD PROTECTION, 2005, 68 (02) :239-245

[5]

Brenner D.J., 1984, BERGEYS MANUAL SYSTE, VI, P408

[6]

Sequence heterogeneities among 16S ribosomal RNA sequences, and their effect on phylogenetic analyses at the species level [J].

Cilia, V ;

Lafay, B ;

Christen, R .

MOLECULAR BIOLOGY AND EVOLUTION, 1996, 13 (03) :451-461

[7]

Strategies for the detection of Escherichia coli O157:H7 in foods [J].

Deisingh, AK ;

Thompson, M .

JOURNAL OF APPLIED MICROBIOLOGY, 2004, 96 (03) :419-429

[8]

EC, 2005, Commission Regulation (EC) No 2073

[9]

European Parliament and Council, 1998, OFF J EUR COMMUNIT L, VL330, P32

[10]

Direct PCR detection of Escherichia coli O157:H7 [J].

Fode-Vaughan, KA ;

Maki, JS ;

Benson, JA ;

Collins, MLP .

LETTERS IN APPLIED MICROBIOLOGY, 2003, 37 (03) :239-243

← 1 2 →