Ion mobility-mass spectrometry reveals long-lived, unfolded intermediates in the dissociation of protein complexes

被引:191
作者
Ruotolo, Brandon T.
Hyung, Suk-Joon
Robinson, Paula M.
Giles, Kevin
Bateman, Robert H.
Robinson, Carol V.
机构
[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
[2] Waters MS Technol Ctr, Manchester M23 9LZ, Lancs, England
关键词
dissociation; electrospray ionization; mass spectrometry; protein structures;
D O I
10.1002/anie.200702161
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Folded or not? Ion mobility-mass spectrometry investigation of an activated macromolecular protein complex lends insight into the structures of intermediates formed in the dissociation process. The activated ions of human tetrameric transthyretin populate partially folded intermediate states (see picture; folded subunits in blue, partially unfolded subunits in red) prior to dissociation. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:8001 / 8004
页数:4
相关论文
共 30 条
[1]   Polydispersity of a mammalian chaperone:: Mass spectrometry reveals the population of oligomers in αB-crystallin [J].
Aquilina, JA ;
Benesch, JLP ;
Bateman, OA ;
Slingsby, C ;
Robinson, CV .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (19) :10611-10616
[2]   Monitoring structural changes of proteins in an ion trap over ∼10-200 ms:: Unfolding transitions in cytochrome c ions [J].
Badman, ER ;
Hoaglund-Hyzer, CS ;
Clemmer, DE .
ANALYTICAL CHEMISTRY, 2001, 73 (24) :6000-6007
[3]   Mass spectrometry of macromolecular assemblies: preservation and dissociation [J].
Benesch, JL ;
Robinson, CV .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2006, 16 (02) :245-251
[4]   Tandem mass spectrometry reveals the quaternary organization of macromolecular assemblies [J].
Benesch, Justin L. P. ;
Aquilina, J. Andrew ;
Ruotolo, Brandon T. ;
Sobott, Frank ;
Robinson, Carol V. .
CHEMISTRY & BIOLOGY, 2006, 13 (06) :597-605
[5]   Amyloid β-protein:: Monomer structure and early aggregation states of Aβ42 and its Pro19 alloform [J].
Bernstein, SL ;
Wyttenbach, T ;
Baumketner, A ;
Shea, JE ;
Bitan, G ;
Teplow, DB ;
Bowers, MT .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (07) :2075-2084
[6]  
Clemmer DE, 1997, J MASS SPECTROM, V32, P577
[7]  
CLEMMER IM, DATABASE
[8]   Thermal decomposition of a gaseous multiprotein complex studied by blackbody infrared radiative dissociation. Investigating the origin of the asymmetric dissociation behavior [J].
Felitsyn, N ;
Kitova, EN ;
Klassen, JS .
ANALYTICAL CHEMISTRY, 2001, 73 (19) :4647-4661
[9]   Structural motifs of DNA complexes in the gas phase [J].
Gidden, J ;
Baker, ES ;
Ferzoco, A ;
Bowers, MT .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2005, 240 (03) :183-193
[10]   Investigation of intact protein complexes by mass spectrometry [J].
Heck, AJR ;
van den Heuvel, RHH .
MASS SPECTROMETRY REVIEWS, 2004, 23 (05) :368-389