Evidence that 5-MT3 receptors in the nucleus tractus solitarius and other brainstem areas modulate the vagal bradycardia evoked by activation of the von Bezold-Jarisch reflex in the anesthetized rat

被引:28
作者
Pires, JGP
Silva, SR
Ramage, AG
Futuro-Neto, HDA
机构
[1] Univ Fed Espirito Santo, Ctr Biomed, Dept Ciencias Fisiol, BR-29040090 Vitoria, ES, Brazil
[2] Royal Free Hosp, Sch Med, Acad Dept Pharmacol, London NW3 2PF, England
关键词
central; 5-HT3; receptor; cardiopulmonary reflex; nucleus tractus solitarius;
D O I
10.1016/S0006-8993(98)00109-7
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The effects of intracisternal (i.c.) application of the 5-HT3 receptor antagonist granisetron (0.016-0.16 mu g kg(-1)) and the agonist phenylbiguanide (0.3-3 mu g kg(-1)) on reflex bradycardia evoked by injection of phenylbiguanide (i.v.; 10 mu g kg(-1)) were investigated in urethane anesthetized atenolol-pretreated rats. The effect of bilateral microinjection of granisetron (10 nmol per side, 100 nl) into the nucleus tractus solitarius (NTS) on the reflex was also investigated. Intracisternal administration of granisetron dose-dependently (0.016-0.16 pg kg(-1)) and significantly attenuated the reflex bradycardia whilst the highest dose given i.v. had no significant effect on the reflex bradycardia. Phenylbiguanide given i.c. only caused significant potentiation at the middle dose (1 mu g kg(-1)), having no significant effects at the other doses. Neither granisetron nor phenylbiguanide given i.c. affected resting heart rate or blood pressure. Granisetron microinjected bilaterally into the NTS also significantly attenuated both reflex bradycardia and hypotension. It is concluded that excitation of cardiac vagal motoneurones evoked by cardiopulmonary afferents involves activation of 5-HT3 receptors located in the nucleus tractus solitarius and other brainstem areas. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:229 / 234
页数:6
相关论文
共 38 条
[31]  
Skinner MR, 1997, J PHYSIOL-LONDON, V501P, pP72
[32]  
Skinner MR, 1997, BRIT J PHARMACOL, V120, pP25
[34]   LABELING OF 5-HT(3) RECEPTOR RECOGNITION SITES IN THE RAT-BRAIN USING THE AGONIST RADIOLIGAND [H-3] META-CHLOROPHENYLBIGUANIDE [J].
STEWARD, LJ ;
WEST, KE ;
KILPATRICK, GJ ;
BARNES, NM .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1993, 243 (01) :13-18
[35]  
STREET JA, 1979, ARCH INT PHARMACOD T, V237, P180
[36]   CENTRAL DISTRIBUTION OF SUBSTANCE-P, CALCITONIN-GENE-RELATED PEPTIDE AND 5-HYDROXYTRYPTAMINE IN VAGAL SENSORY AFFERENTS IN THE RAT DORSAL MEDULLA [J].
SYKES, RM ;
SPYER, KM ;
IZZO, PN .
NEUROSCIENCE, 1994, 59 (01) :195-210
[37]   In vivo effects of 5-hydroxytryptamine receptor activation on rat nucleus tractus solitarius neurones excited by vagal C-fibre afferents [J].
Wang, Y ;
Ramage, AG ;
Jordan, D .
NEUROPHARMACOLOGY, 1997, 36 (4-5) :489-498
[38]  
Wang Y, 1997, BRIT J PHARMACOL, V120, pP38