IL-12 and TNF-alpha production by dendritic cells (DCs) is a critical step in the initiation of local inflammation and adaptive immune responses. We show in this study that a small molecule immune response modifier that is a Toll-like receptor 7 (TLR7) agonist induces IL-12 and TNF-alpha production from murine CD11c(+)CD11b(+)CD8(-) DCs, a subset not previously known for this activity. Stimulation of these DCs through TLR7 in vivo induces significant cytokine production even 12 h after initial stimulation, as wen as migration of the DC into T cell zones of the lymphoid tissue. In contrast, stimulation through TLR4 and TLR9 induced IL-12 production predominantly from CD8(+) DCs, consistent with previously published data. All TLR stimuli induced the increase in surface expression of the activation markers B7-1, B7-2, and class II in both CD8(+) and CD8(-) DCs, demonstrating that CD8(+) DCs do respond to TLR7-mediated stimuli. To date this is the only known stimuli to induce preferential cytokine production from CD8(-) DCs. Given the efficacy of TLR7 agonists as antiviral agents, the data collectively indicate that stimulation of CD8(-) DCs through TLR7 most likely plays a role in the generation of antiviral immune responses.
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Belz, GT
Behrens, GMN
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Behrens, GMN
Smith, CM
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Smith, CM
Miller, JFAP
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Miller, JFAP
Jones, C
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Jones, C
Lejon, K
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Lejon, K
Fathman, CG
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Fathman, CG
Mueller, SN
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Mueller, SN
Shortman, K
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Shortman, K
Carbone, FR
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Carbone, FR
Heath, WR
论文数: 0引用数: 0
h-index: 0
机构:
PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, AustraliaPO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Belz, GT
Behrens, GMN
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Behrens, GMN
Smith, CM
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Smith, CM
Miller, JFAP
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Miller, JFAP
Jones, C
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Jones, C
Lejon, K
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Lejon, K
Fathman, CG
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Fathman, CG
Mueller, SN
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Mueller, SN
Shortman, K
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Shortman, K
Carbone, FR
论文数: 0引用数: 0
h-index: 0
机构:PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia
Carbone, FR
Heath, WR
论文数: 0引用数: 0
h-index: 0
机构:
PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, AustraliaPO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Immunol, Parkville, Vic 3050, Australia