Alterations of PLCβ1 in mouse eggs change calcium oscillatory behavior following fertilization

被引：66

作者：

Tgarashi, Hideki ^{[2
]}

Knott, Jason G. ^{[2
]}

SchultZ, Richard M. ^{[2
,3
]}

Williams, Carnien J. ^{[1
,2
]}

机构：

[1] Univ Penn, Dept Obstet & Gynecol, Philadelphia, PA 19104 USA

[2] Univ Penn, Ctr Res Reprod & Womens Hlth, Philadelphia, PA 19104 USA

[3] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

来源：

DEVELOPMENTAL BIOLOGY | 2007年 / 312卷 / 01期

关键词：

egg activation; calcium; phospholipase C; RNAi; fertilization; mouse;

D O I：

10.1016/j.ydbio.2007.09.028

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Inositol 1,4,5-trisphosphate generated by the action of a phospholipase C (PLC) mediates release of intracellular Ca2+ that is essential for sperm-induced activation of mammalian eggs. Much attention currently focuses on the role of sperm-derived PLC in generating changes in egg intracellular Ca2+ despite the fact that PLC zeta constitutes a very small fraction of the total amount of PLC in a fertilized egg. Eggs express several isoforms of PLC, but a role for an egg-derived PLC in sperm-induced Ca2+ oscillations has not been examined. Reducing egg PLC beta 1 I by a transgenic RNAi approach resulted in a significant decrease in Ca2+ transient amplitude, but not duration or frequency, following insemination. Furthermore, overexpressing PLC beta 1 by microinjecting a Plc beta 1 cRNA significantly perturbed the duration and frequency of Ca2+ transients and disrupted the characteristic shape of the first transient. These results provide the first evidence for a role of an egg-derived PLC acting in conjunction with a sperm-derived PLC zeta in egg activation. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：321 / 330

页数：10

共 46 条

[1] Cytoplasmic and nuclear phospholipase C-β1 relocation:: Role in resumption of meiosis in the mouse oocyte [J].

Avazeri, N ;

Courtot, AM ;

Pesty, A ;

Duquenne, C ;

Lefèvre, B .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (12) :4369-4380

[2] Crucial role of phospholipase CE in chemical carcinogen-induced skin tumor development [J].

Bai, YF ;

Edamatsu, H ;

Maeda, S ;

Saito, H ;

Suzuki, N ;

Satoh, T ;

Kataoka, T .

CANCER RESEARCH, 2004, 64 (24) :8808-8810

[3] ADENYLATE-CYCLASE ACTIVITY IN ZONA-FREE MOUSE OOCYTES [J].

BORNSLAEGER, EA ;

SCHULTZ, RM .

EXPERIMENTAL CELL RESEARCH, 1985, 156 (01) :277-281

[4]

CHATOT CL, 1989, J REPROD FERTIL, V86, P679, DOI 10.1530/jrf.0.0860679

[5] Egg-to-embryo transition is driven by differential responses to Ca2+ oscillation number [J].

Ducibella, T ;

Huneau, D ;

Angelichio, E ;

Xu, Z ;

Schultz, RM ;

Kopf, GS ;

Fissore, R ;

Madoux, S ;

Ozil, JP .

DEVELOPMENTAL BIOLOGY, 2002, 250 (02) :280-291

[6] Phospholipase C in mouse oocytes: Characterization of beta and gamma isoforms and their possible involvement in sperm-induced Ca2+ spiking [J].

Dupont, G ;

McGuinness, OM ;

Johnson, MH ;

Berridge, MJ ;

Borgese, F .

BIOCHEMICAL JOURNAL, 1996, 316 :583-591

[7] Requirement of phospholipase Cδ4 for the zona pellucida-induced acrosome reaction [J].

Fukami, K ;

Nakao, K ;

Inoue, T ;

Kataoka, Y ;

Kurokawa, M ;

Fissore, RA ;

Nakamura, K ;

Katsuki, M ;

Mikoshiba, K ;

Yoshida, N ;

Takenawa, T .

SCIENCE, 2001, 292 (5518) :920-923

[8] Cutting edge:: Essential role of phospholipase C-γ2 in B cell development and function [J].

Hashimoto, A ;

Takeda, K ;

Inaba, M ;

Sekimata, M ;

Kaisho, T ;

Ikehara, S ;

Homma, Y ;

Akira, S ;

Kurosaki, T .

JOURNAL OF IMMUNOLOGY, 2000, 165 (04) :1738-1742

[9]

JONES KT, 1995, DEVELOPMENT, V121, P3259

[10]

Jones KT, 1998, DEVELOPMENT, V125, P4627

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