Alterations of PLCβ1 in mouse eggs change calcium oscillatory behavior following fertilization

被引：66

作者：

Tgarashi, Hideki ^{[2
]}

Knott, Jason G. ^{[2
]}

SchultZ, Richard M. ^{[2
,3
]}

Williams, Carnien J. ^{[1
,2
]}

机构：

[1] Univ Penn, Dept Obstet & Gynecol, Philadelphia, PA 19104 USA

[2] Univ Penn, Ctr Res Reprod & Womens Hlth, Philadelphia, PA 19104 USA

[3] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

来源：

DEVELOPMENTAL BIOLOGY | 2007年 / 312卷 / 01期

关键词：

egg activation; calcium; phospholipase C; RNAi; fertilization; mouse;

D O I：

10.1016/j.ydbio.2007.09.028

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Inositol 1,4,5-trisphosphate generated by the action of a phospholipase C (PLC) mediates release of intracellular Ca2+ that is essential for sperm-induced activation of mammalian eggs. Much attention currently focuses on the role of sperm-derived PLC in generating changes in egg intracellular Ca2+ despite the fact that PLC zeta constitutes a very small fraction of the total amount of PLC in a fertilized egg. Eggs express several isoforms of PLC, but a role for an egg-derived PLC in sperm-induced Ca2+ oscillations has not been examined. Reducing egg PLC beta 1 I by a transgenic RNAi approach resulted in a significant decrease in Ca2+ transient amplitude, but not duration or frequency, following insemination. Furthermore, overexpressing PLC beta 1 by microinjecting a Plc beta 1 cRNA significantly perturbed the duration and frequency of Ca2+ transients and disrupted the characteristic shape of the first transient. These results provide the first evidence for a role of an egg-derived PLC acting in conjunction with a sperm-derived PLC zeta in egg activation. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：321 / 330

页数：10

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