Identification of pollen-expressed pectin methylesterase inhibitors in Arabidopsis

被引：84

作者：

Wolf, S ^{[1
]}

Grsic-Rausch, S ^{[1
]}

Rausch, T ^{[1
]}

Greiner, S ^{[1
]}

机构：

[1] Heidelberg Inst Plant Sci, HIP, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 2003年 / 555卷 / 03期

关键词：

pectin methylesterase; plant cell wall; post-translational regulation; functional genomics; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(03)01344-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pectin methylesterases (PMEs) play an essential role during plant development by affecting the mechanical properties of the plant cell wall. Previous work indicated that plant PMEs may be subject to post-translational regulation. Here, we report the analysis of two proteinaceous inhibitors of PME in Arabidopsis thaliana (AtPMEI1 and 2). The functional analysis of recombinant AtPMEI1 and 2 proteins revealed that both proteins are able to inhibit PME activity from flowers and siliques. Quantitative RT-PCR analysis indicated that expression of AtPMEI1 and 2 mRNAs is tightly regulated during plant development with highest mRNA levels in flowers. Promotor::GUS fusions demonstrated that expression is mostly restricted to pollen. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：551 / 555

页数：5

共 23 条

[1] Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants [J].

Brummell, DA ;

Harpster, MH .

PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) :311-340

[2] Kiwi protein inhibitor of pectin methylesterase - Amino-acid sequence and structural importance of two disulfide bridges [J].

Camardella, L ;

Carratore, V ;

Ciardiello, MA ;

Servillo, L ;

Balestrieri, C ;

Giovane, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (14) :4561-4565

[3] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[4] Cloning of a tobacco apoplasmic invertase inhibitor - Proof of function of the recombinant protein and expression analysis during plant development [J].

Greiner, S ;

Krausgrill, S ;

Rausch, T .

PLANT PHYSIOLOGY, 1998, 116 (02) :733-742

[5] Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers [J].

Greiner, S ;

Rausch, T ;

Sonnewald, U ;

Herbers, K .

NATURE BIOTECHNOLOGY, 1999, 17 (07) :708-711

[6]

Greiner S, 2000, AUST J PLANT PHYSIOL, V27, P807

[7] Phytochelatin synthase genes from arabidopsis and the yeast Schizosaccharomyces pombe [J].

Ha, SB ;

Smith, AP ;

Howden, R ;

Dietrich, WM ;

Bugg, S ;

O'Connell, MJ ;

Goldsbrough, PB ;

Cobbett, CS .

PLANT CELL, 1999, 11 (06) :1153-1163

[8] MOLECULAR-CLONING OF TOMATO PECTIN METHYLESTERASE GENE AND ITS EXPRESSION IN RUTGERS, RIPENING INHIBITOR, NONRIPENING, AND NEVER RIPE TOMATO FRUITS [J].

HARRIMAN, RW ;

TIEMAN, DM ;

HANDA, AK .

PLANT PHYSIOLOGY, 1991, 97 (01) :80-87

[9]

HOTHORN M, 2003, IN PRESS J MOL BIOL

[10]

HOTHORN M, 2003, IN PRESS ACTA CRYSTA

← 1 2 3 →