Identification of pollen-expressed pectin methylesterase inhibitors in Arabidopsis

被引：84

作者：

Wolf, S ^{[1
]}

Grsic-Rausch, S ^{[1
]}

Rausch, T ^{[1
]}

Greiner, S ^{[1
]}

机构：

[1] Heidelberg Inst Plant Sci, HIP, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 2003年 / 555卷 / 03期

关键词：

pectin methylesterase; plant cell wall; post-translational regulation; functional genomics; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(03)01344-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pectin methylesterases (PMEs) play an essential role during plant development by affecting the mechanical properties of the plant cell wall. Previous work indicated that plant PMEs may be subject to post-translational regulation. Here, we report the analysis of two proteinaceous inhibitors of PME in Arabidopsis thaliana (AtPMEI1 and 2). The functional analysis of recombinant AtPMEI1 and 2 proteins revealed that both proteins are able to inhibit PME activity from flowers and siliques. Quantitative RT-PCR analysis indicated that expression of AtPMEI1 and 2 mRNAs is tightly regulated during plant development with highest mRNA levels in flowers. Promotor::GUS fusions demonstrated that expression is mostly restricted to pollen. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：551 / 555

页数：5