Expression of DJ-1 in the adult mouse CNS

被引：57

作者：

Badera, V

Zhu, XR

Lübbert, H

Stichel, CC

机构：

[1] Ruhr Univ Bochum, Dept Anim Physiol, D-44780 Bochum, Germany

[2] Biofrontera Pharmaceut AG, D-51377 Leverkusen, Germany

来源：

BRAIN RESEARCH | 2005年 / 1041卷 / 01期

关键词：

parkinsonism; DJ-1; expression pattem; in situ hybridization; immunohistochemistry; mouse;

D O I：

10.1016/j.brainres.2005.02.006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Recessively inherited mutations in DJ-1 have recently been linked to familial fortes of parkinsonism. However, the regional and cellular expression of DJ-1 is largely unknown. In the present study, we mapped the distribution of DJ-1 transcript and protein by non-radioactive in situ hybridization and immunohistochemistry in adult mouse CNS. For immunohistochemical analysis, we raised it polyclonal antiserum against GST-mouse DJ-1 fusion protein. DJ-1 transcript and protein were strongly and homogenously expressed in all CNS regions. Colocalization experiments revealed expression in neurons of different neurotransmitter phenotypes and in all glial cell type, such its astrocytes, microglia and oligodendrocytes. DJ-1 protein was diffusely distributed throughout the cytoplasma of the soma and the proximal parts of the processes, while the nuclei were always spared. The high expression of DJ-1 in neuronal and filial cells, that is not confined to a single functional system or any anatomical area, supports the view of a basic physiological role in cell biology. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：102 / 111

页数：10

共 34 条

[1] The role of pathogenic DJ-1 mutations in Parkinson's disease [J].

Abou-Sleiman, PM ;

Healy, DG ;

Quinn, N ;

Lees, AJ ;

Wood, NW .

ANNALS OF NEUROLOGY, 2003, 54 (03) :283-286

[2] The expression of DJ-1 (PARK7) in normal human CNS and idiopathic Parkinson's disease [J].

Bandopadhyay, R ;

Kingsbury, AE ;

Cookson, MR ;

Reid, AR ;

Evans, IM ;

Hope, AD ;

Pittman, AM ;

Lashley, T ;

Canet-Aviles, R ;

Miller, DW ;

McLendon, C ;

Strand, C ;

Leonard, AJ ;

Abou-Sleiman, PM ;

Healy, DG ;

Ariga, H ;

Wood, NW ;

de Silva, R ;

Revesz, T ;

Hardy, JA ;

Lees, AJ .

BRAIN, 2004, 127 :420-430

[3] Dimerization of Parkinson's disease - causing DJ-1 and formation of high molecular weight complexes in human brain [J].

Baulac, S ;

LaVoie, MJ ;

Strahle, J ;

Schlossmacher, MG ;

Xia, WM .

MOLECULAR AND CELLULAR NEUROSCIENCE, 2004, 27 (03) :236-246

[4] Quantitative cytochemical mapping of mitochondrial enzymes in rat cerebella [J].

Bertoni-Freddari, C ;

Fattoretti, P ;

Casoli, T ;

Di Stefano, G ;

Solazzi, M ;

Meier-Ruge, W .

MICRON, 2001, 32 (04) :405-410

[5] Linking DJ-1 to neurodegeneration offers novel insights for understanding the pathogenesis of Parkinson's disease [J].

Bonifati, V ;

Oostra, BA ;

Heutink, P .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2004, 82 (03) :163-174

[6] Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism [J].

Bonifati, V ;

Rizzu, P ;

van Baren, MJ ;

Schaap, O ;

Breedveld, GJ ;

Krieger, E ;

Dekker, MCJ ;

Squitieri, F ;

Ibanez, P ;

Joosse, M ;

van Dongen, JW ;

Vanacore, N ;

van Swieten, JC ;

Brice, A ;

Meco, G ;

van Duijn, CM ;

Oostra, BA ;

Heutink, P .

SCIENCE, 2003, 299 (5604) :256-259

[7]

Braak H, 2000, J NEUROL, V247, P3

[8] Rare genetic mutations shed light on the pathogenesis of Parkinson disease [J].

Dawson, TM ;

Dawson, VL .

JOURNAL OF CLINICAL INVESTIGATION, 2003, 111 (02) :145-151

[9] Neuropathology of Parkinson's disease [J].

Forno, LS .

JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1996, 55 (03) :259-272

[10] Early-onset Parkinson's disease caused by a compound heterozygous DJ-1 mutation [J].

Hague, S ;

Rogaeva, E ;

Hernandez, D ;

Gulick, C ;

Singleton, A ;

Hanson, M ;

Johnson, J ;

Weiser, R ;

Gallardo, M ;

Ravina, B ;

Gwinn-Hardy, K ;

Crawley, A ;

St George-Hyslop, PH ;

Lang, AE ;

Heutink, P ;

Bonifati, V ;

Hardy, J ;

Singleton, A .

ANNALS OF NEUROLOGY, 2003, 54 (02) :271-274

← 1 2 3 4 →