Characterisation of Leishmania donovani promastigotes resistant to hexadecylphosphocholine (miltefosine)

被引：138

作者：

Seifert, K

Matu, S

Pérez-Victoria, FJ

Castanys, S

Gamarro, F

Croft, SL

机构：

[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England

[2] CSIC, Inst Parasitol & Biomed Lopez Neyra, Granada, Spain

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2003年 / 22卷 / 04期

关键词：

Leishmania; hexadecylphosphocholine; miltefosine; drug resistance;

D O I：

10.1016/S0924-8579(03)00125-0

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Leishmania donovani promastigote lines resistant to hexadecylphosphocholine (HePC, miltefosine) at 2.5, 5.0, 10.0, 20.0 and 40.0 muM were developed in vitro by continuous step-wise drug pressure. The 40 muM line was 15 times more resistant to HePC than the wild-type clone and showed cross-resistance to the ether lipid ET-18-OCH3 (edelfosine) but not to the standard anti-leishmanial drugs. Resistance was stable up to 12 weeks in drug-free culture medium. No amplification of specific genes, including the multidrug resistance P-glycoprotein gene, could be detected in the resistant parasites. (C) 2003 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：380 / 387

页数：8

共 42 条

[1] CYTOTOXICITY OF ESTER AND ETHER LYSOPHOSPHOLIPIDS ON LEISHMANIA-DONOVANI PROMASTIGOTES [J].