Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides

被引：17

作者：

Brown, LJ

May, JP

Brown, T ^{[1
]}

机构：

[1] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Southampton, Oswel Res Prod, Southampton SO16 7PX, Hants, England

来源：

TETRAHEDRON LETTERS | 2001年 / 42卷 / 13期

关键词：

D O I：

10.1016/S0040-4039(01)00224-6

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2587 / 2591

页数：5

共 17 条

[1]

BROWN T, 2000, PATENT PENDING

[2]

Brown T., 1991, Oligonucleotides and analogues. A Practical Approach, P1

[3] OBSERVING THE HELICAL GEOMETRY OF DOUBLE-STRANDED DNA IN SOLUTION BY FLUORESCENCE RESONANCE ENERGY-TRANSFER [J].