SYNTHESIS AND ANTIBODY-MEDIATED DETECTION OF 2,4-DINITROPHENYL (DNP) LABELED OLIGONUCLEOTIDES

被引：6

作者：

DAVISON, A

DUCKWORTH, G

RAO, MV

MCCLEAN, J

GRZYBOWSKI, J

POTIER, P

BROWN, T

CUBIE, H

机构：

[1] CRUACHEM LTD,GLASGOW,LANARK,SCOTLAND

[2] UNIV EDINBURGH,DEPT CHEM,EDINBURGH,MIDLOTHIAN,SCOTLAND

[3] CITY HOSP,REG VIRUS LAB,EDINBURGH,MIDLOTHIAN,SCOTLAND

来源：

NUCLEOSIDES & NUCLEOTIDES | 1995年 / 14卷 / 3-5期

关键词：

D O I：

10.1080/15257779508012531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Different DNP phosphoramidites based on non-nucleoside and nucleoside backbone molecules are developed and used in the multiple labelling of oligonucleotides during the solid phase synthesis. It is demonstrated that the antibody mediated detection of DNP labelled oligonucleotides is comparable to that of digoxigenin, biotin and fluorescein.

引用

页码：1049 / 1052

页数：4

共 7 条

[1]

CUBIE H, COMMUNICATION

[2] SYNTHESIS AND ANTIBODY-MEDIATED DETECTION OF OLIGONUCLEOTIDES CONTAINING MULTIPLE 2,4-DINITROPHENYL REPORTER GROUPS [J].